91288-93-6Relevant articles and documents
SYNTHESIS OF DNA FRAGMENTS BY THE HYDROXYBENZOTRIAZOLE PHOSPHOTRIESTER APPROACH
Marugg, J. E.,Tromp, M.,Jhurani, P.,Hoyng, C. F.,Marel, G. A. van der,Boom, J. H. van
, p. 73 - 78 (2007/10/02)
The application of the phosphorylating agent 2-chlorophenyl-o,o-bis--phosphate (HOBT-approach) for the introduction of 3'-5'-phosphotriester linkages between a properly 5'-protected (4,4'-dimethoxytrityl) deoxy-nucleoside and a deoxy-nucleoside having free OH groups is described.In this way, all sixteen partially-protected DNA dimers having a free 3'-OH group have been synthesized.The dimers thus obtained have been applied, using the HOBT-approach, to the synthesis of the DNA fragments d-CGCGCG, d-CGTACG and d-ATATTAATAAC.