The effect of the presence of RNA upon Diethyl pyrocarbonate (cas 1609-47-8) inhibition of ribonuclease
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Add time:07/23/2019 Source:sciencedirect.com
The relationship of RNA, ribonuclease and diethylpyrocarbonate has been investigated using various concentrations of DEP, with and without the simultaneous addition of RNA.It has been found: 1.1. The presence of any considerable amount of RNA interferes with the inhibition of ribonuclease by Diethyl pyrocarbonate (cas 1609-47-8).2.2. The results do not appear to be explained by a direct interaction between diethyl pyrocarbonate and RNA.A question is raised as to the utility of diethyl pyrocarbonate as a ribonuclease inhibitor, if free RNA is present in the solution being extracted.
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