Research articleEnzymatic esterification of Oleic acid (cas 112-80-1) by Candida rugosa lipase immobilized onto biochar

Add time:08/27/2019    Source:sciencedirect.com

The immobilization of Candida rugosa lipase (CRL) onto biochar was studied in a series of batch experiments. CRL sorption behavior was evaluated as a function of pH, enzyme concentration, temperature and ionic strength. As the immobilized lipase was used for the catalytic esterification of Oleic acid (cas 112-80-1), its resistance to solvents and thermal stability were evaluated. CRL adsorption increased by increasing temperature, and with higher pH, reaching a maximum at pH 7.0. Immobilization increased lipase stability at 40 °C by more than 80% when compared to the free enzyme. Moreover, immobilized CRL showed high stability in the presence of tert-butanol, which prevents premature deactivation of the enzyme caused by alcohols during the reaction. Immobilization of CRL increased the oleic acid conversion rate. Our results suggest that biochar is a highly promising material for the immobilization of CRL lipase for the catalytic production of esters.

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