Effects of metal ions on the catalytic degradation of Dicofol (cas 115-32-2) by cellulase
-
Add time:08/28/2019 Source:sciencedirect.com
A new technique whereby cellulase immobilized on aminated silica was applied to catalyze the degradation of Dicofol (cas 115-32-2), an organochlorine pesticide. In order to evaluate the performance of free and immobilized cellulase, experiments were carried out to measure the degradation efficiency. The Michaelis constant, Km, of the reaction catalyzed by immobilized cellulase was 9.16 mg/L, and the maximum reaction rate, Vmax, was 0.40 mg/L/min, while that of free cellulase was Km = 8.18 mg/L, and Vmax = 0.79 mg/L/min, respectively. The kinetic constants of catalytic degradation were calculated to estimate substrate affinity. Considering that metal ions may affect enzyme activity, the effects of different metal ions on the catalytic degradation efficiency were explored. The results showed that the substrate affinity decreased after immobilization. Monovalent metal ions had no effect on the reaction, while divalent metal ions had either positive or inhibitory effects, including activation by Mn2 +, reversible competition with Cd2 +, and irreversible inhibition by Pb2 +. Ca2 + promoted the catalytic degradation of dicofol at low concentrations, but inhibited it at high concentrations. Compared with free cellulase, immobilized cellulase was affected less by metal ions. This work provided a basis for further studies on the co-occurrence of endocrine-disrupting chemicals and heavy metal ions in the environment.
We also recommend Trading Suppliers and Manufacturers of Dicofol (cas 115-32-2). Pls Click Website Link as below: cas 115-32-2 suppliers
Prev:Deciphering the toxic effects of organochlorine pesticide, Dicofol (cas 115-32-2) on human RBCs and lymphocytes
Next:Removal of Dicofol (cas 115-32-2) from Waste-Water Using Advanced Oxidation Process☆) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Detection of Dicofol (cas 115-32-2) and related pesticides in human breast milk from China, Korea and Japan08/31/2019
- Theoretical studies on the OH-initiated photodegradation mechanism of Dicofol (cas 115-32-2)08/30/2019
- Removal of Dicofol (cas 115-32-2) from Waste-Water Using Advanced Oxidation Process☆08/29/2019
- Deciphering the toxic effects of organochlorine pesticide, Dicofol (cas 115-32-2) on human RBCs and lymphocytes08/27/2019
- Uptake and depuration kinetics of Dicofol (cas 115-32-2) metabolite 4,4′-dichlorobenzophenone, in the edible Asiatic clam Meretrix meretrix08/26/2019
- Reaction mechanism of Dicofol (cas 115-32-2) removal by cellulase08/25/2019
- Sonochemical degradation of endocrine-disrupting organochlorine pesticide Dicofol (cas 115-32-2): Investigations on the transformation pathways of dechlorination and the influencing operating parameters08/24/2019
- Mechanism and kinetics studies of the atmospheric oxidation of p,p'-Dicofol (cas 115-32-2) by OH and NO3 radicals08/23/2019
- Hydrodynamic cavitation assisted degradation of persistent endocrine-disrupting organochlorine pesticide Dicofol (cas 115-32-2): Optimization of operating parameters and investigations on the mechanism of intensification08/22/2019