- Synthesis, two-photon absorption properties and bioimaging applications of mono-, di- and hexa-branched pyrimidine derivatives
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A series of mono-, di- and hexa-branched pyrimidine derivatives possessing two-photon absorption have been synthesized. The spectral features of the dyes showed solvatochromic with significant bathochromic shifting of the emission spectra and large Stokes shifts in more polar solvents mainly due to the intramolecular charge-transfer. The two-photon absorption cross-section values were measured by two-photon excited fluorescence and open aperture Z-scan methods. The results revealed that the two-photon absorption cross-section values were enhanced with increasing electron-donating strength of the end group and branch number. The photophysical properties of the dyes were further investigated with the aid of theoretical calculations. In addition, the di-branched dyes has the largest values of two-photon absorption cross-section per molecular weight, low cytotoxicity and can be internalized by human liver cancer cell line and accumulates in the cytoplasm, allowing for live cell two-photon fluorescence imaging, so it is promising for in vitro and vivo cellular imaging applications.
- Wang, Hui,Zhang, Qiong,Zhang, Jun,Li, Lin,Zhang, Qian,Li, Shengli,Zhang, Shengyi,Wu, Jieying,Tian, Yupeng
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- Detection living body Fe2 + Double-photon fluorescence probe as well as preparation method and application thereof
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The invention relates to a pyrimidine-based dye which can be quickly regulated and controlled by light. Sensitive detection living body Fe2 + A double-photon fluorescence probe belongs to the field of organic fluorescent probes. The fluorescent probe is PyFe fluorescence probe, and the structural formula of PyFe fluorescence probe is as shown in formula (I). Fe In vivo can be accurately detected by the fluorescent probe. 2 + , Interference of other ionic amino acids in the cells is avoided. The probe has very fast response rate and high ion selectivity, can realize naked eye detection in vitro and also has good biocompatibility. The fluorescence confocal imaging experiment shows that the probe has good cell permeability, does not have toxic or side effects on cells and organisms, and has dual photonicity, so that the depth imaging of living animals or tissues can be realized.
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- Synthesis, optical properties and singlet oxygen generation of a phthalocyanine derivative containing strong two-photon-absorbing chromophores in the periphery
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A phthalocyanine derivative containing two-photon chromophores which are based on pyrimidine was designed and synthesized. Its light-emission property was studied in detail, and a strong energy transfer from peripheral chromophores to the phthalocyanine c
- Liu, Zijun,Xiong, Xiaoqin,Li, Yu,Li, Suyue,Qin, Jingui
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scheme or table
p. 1804 - 1809
(2012/05/20)
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- Two-photon absorption enhancement induced by aggregation due to intermolecular hydrogen bonding in V-shaped 2-hydroxypyrimidine derivatives
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The aggregation of V-shaped hydroxypyrimidine derivatives is formed through an intermolecular hydrogen bond in chloroform that cannot form a hydrogen bond with the solute, giving rise to a large enhancement of the two-photon absorption and two-photon induced fluorescence. The Royal Society of Chemistry.
- Liu, Zijun,Shao, Pin,Huang, Zhenli,Liu, Bo,Chen, Tao,Qin, Jingui
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supporting information; experimental part
p. 2260 - 2262
(2009/02/04)
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- Two-photon absorption of a series of V-shape molecules: The influence of acceptor's strength on two-photon absorption in a noncentrosymmetric D-π-A-π-D system
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A series of V-shape D-π-A-π-D molecules were synthesized based on pyrimidine as the acceptor. The strength of the acceptor (A) in these molecules was systematically adjusted through changing the substituent group in pyrimidine. Their two-photon absorption properties were measured by two-photon induced fluorescence techniques with a mode-locked Ti:sapphire laser (100 fs, 80 MHz) in the range of 720 to 880 nm. The results indicate that the strength of the acceptor has an important influence on the two-photon absorption properties of this noncentrosymmetric D-π-A-π-D system, and a molecule with a strong acceptor is liable to display a large two-photon absorption cross-section. The Royal Society of Chemistry.
- Liu, Zijun,Chen, Tao,Liu, Bo,Huang, Zhen-Li,Huang, Ting,Li, Suyue,Xu, Yuxi,Qin, Jingui
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experimental part
p. 4685 - 4689
(2009/09/25)
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