10019-03-1

Basic information

• Product Name: NAPHTHOL AS-BS PHOSPHATE
• Synonyms: NAPHTHOL AS-BS PHOSPHATE;N-(3-nitrophenyl)-3-(phosphonooxy)naphthalene-2-carboxamide;NAPHTHOL AS-BS-PHOSPHATE FOR &;N-(3-Nitrophenyl)-3-(phosphonooxy)-2-naphthalenecarboxamide;Einecs 233-014-2
• CAS NO: 10019-03-1
• Molecular Formula: C₁₇H₁₃N₂O₇P
• Molecular Weight: 388.27
• EINECS: 233-014-2
• Mol File: 10019-03-1.mol

Chemical Properties

• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: 1.611g/cm³
• Refractive Index: 1.74
• Storage Temp.: 2-8°C
• PKA: 1.03±0.30(Predicted)
• CAS DataBase Reference: NAPHTHOL AS-BS PHOSPHATE(CAS DataBase Reference)
• NIST Chemistry Reference: NAPHTHOL AS-BS PHOSPHATE(10019-03-1)
• EPA Substance Registry System: NAPHTHOL AS-BS PHOSPHATE(10019-03-1)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• Hazardous Substances Data: 10019-03-1(Hazardous Substances Data)

Usage

Uses

Used in Biochemical Assays:
Naphthol AS-BS phosphate is used as a substrate for detecting and quantifying alkaline phosphatase activity, enabling the study of enzyme kinetics and the diagnosis of diseases related to abnormal enzyme levels.
Used in Enzyme-Linked Immunosorbent Assays (ELISAs):
In ELISAs, Naphthol AS-BS phosphate serves as a colorimetric indicator, allowing for the detection and quantification of specific antigens or antibodies, which is crucial for biomedical research and diagnostic applications.
Used in Biomedical Research:
Naphthol AS-BS phosphate is utilized in various research applications to study the activity and function of alkaline phosphatase, contributing to a better understanding of enzyme mechanisms and their role in disease processes.
Used in Diagnostic Applications:
In the medical field, Naphthol AS-BS phosphate is employed for diagnosing diseases associated with changes in alkaline phosphatase levels, such as liver or bone disorders, by providing a quantitative measure of enzyme activity.
Safety Precautions:
Naphthol AS-BS phosphate should be handled and disposed of with care, as it can cause irritation to the eyes, skin, and respiratory tract, and may be harmful if ingested or inhaled. Proper safety measures, including the use of personal protective equipment and adherence to disposal protocols, are essential to minimize potential health risks.

InChI:InChI=1/C17H13N2O7P/c20-17(18-13-6-3-7-14(10-13)19(21)22)15-8-11-4-1-2-5-12(11)9-16(15)26-27(23,24)25/h1-10H,(H,18,20)(H2,23,24,25)

Upstream product

• 135-65-9 3-hydroxy-3'-nitro-2-naphthanilide 
• 99-09-2 3-nitro-aniline 
• 92-70-6 3-Hydroxy-2-naphthoic acid 

Relevant articles and documents

Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids

A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.