A dipicolinic acid tag for rigid lanthanide tagging of proteins and paramagnetic NMR spectroscopy
A new lanthanide tag was designed for site-specific labeling of proteins with paramagnetic lanthanide ions. The tag, 4-mercaptomethyl-dipicolinic acid, binds lanthanide ions with nanomolar affinity, is readily attached to proteins via a disulfide bond, an
Su, Xun-Cheng,Man, Bradley,Beeren, Sophie,Liang, Haobo,Simonsen, Shane,Schmitz, Christophe,Huber, Thomas,Messerle, Barbara A.,Otting, Gottfried