Chirally-modified oligonucleotides and the control of gene expression. The case of L-DNAs and -RNAs
The affinity of L-DNAs, L-RNAs and LD-DNAs for homopurine · homopyrimidine d.s. D-DNA and s.s. D-RNA was probed by gel electrophoresis and CD spectroscopy. It was found that the L-modified oligomers do not bind to d.s. DNA and to natural RNA that contains all four natural bases. Thus they cannot be used, in general, for the control of gene expression according to the antigene and antisense methodologies. Heterochiral complexes with 1:1 stoichiometry and low hem stability are formed, instead, by homopurinic L- RNA or L/D-DNA and homopyrimidinic L-RNA with the W/C complementary natural RNA sequences.
Garbesi, Anna,Capobianco, Massimo L.,Colonna, Francesco P.,Maffini, Mauro,Niccolai, Daniela,Tondelli, Luisa
Synthesis of the mirror image of the RNA fragment D-CAAGG: A model compound to study interactions between oligonucleotides of opposite handedness
The pentamer L-CAAGG was prepared starting from properly protected β-L-ribonucleosides via a phosphotriester approach.The identity and chiral purity of the pentamer were established by 1H NMR and CD spectroscopy, respectively.
Visser, G. M.,van Westrenen, J.,van Boeckel, C. A. A.,van Boom, J. H.
p. 528 - 537
(2007/10/02)
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