Chirally-modified oligonucleotides and the control of gene expression. The case of L-DNAs and -RNAs

Article abstract of DOI:10.1080/07328319808004239

The affinity of L-DNAs, L-RNAs and LD-DNAs for homopurine · homopyrimidine d.s. D-DNA and s.s. D-RNA was probed by gel electrophoresis and CD spectroscopy. It was found that the L-modified oligomers do not bind to d.s. DNA and to natural RNA that contains all four natural bases. Thus they cannot be used, in general, for the control of gene expression according to the antigene and antisense methodologies. Heterochiral complexes with 1:1 stoichiometry and low hem stability are formed, instead, by homopurinic L- RNA or L/D-DNA and homopyrimidinic L-RNA with the W/C complementary natural RNA sequences.

Full text of DOI:10.1080/07328319808004239

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Chirally-Modifiedoligonucleotides and
the Control of Gene Expression. The
Case of L-DNAS And-RNAS
Anna Garbesi a , Massimo L. Capobianco a , Francesco P. Colonna a ,
Mauro Maflini a , Danieia Niccoiai a & Luisa Tondelli a
a ICoCEA, Consiglio Nazionale delle Ricerche , Via Piero Gobetti 101,
Bologna, 40129, Italy
Published online: 21 Aug 2006.
To cite this article: Anna Garbesi , Massimo L. Capobianco , Francesco P. Colonna , Mauro Maflini ,
Danieia Niccoiai & Luisa Tondelli (1998) Chirally-Modifiedoligonucleotides and the Control of Gene
Expression. The Case of L-DNAS And-RNAS, Nucleosides and Nucleotides, 17:7, 1275-1287, DOI:
10.1080/07328319808004239
To link to this article: http://dx.doi.org/10.1080/07328319808004239
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