- Stereoselective synthesis of γ-hydroxy-α-amino acids through aldolase-transaminase recycling cascades
-
Efficient bi-enzymatic cascades combining aldolases and α-transaminases were designed for the synthesis of γ-hydroxy-α-amino acids. These recycling cascades provide high stereoselectivity, atom economy, and an equilibrium shift of the transamination. l-syn or anti-4-hydroxyglutamic acid and d-anti-4,5-dihydroxynorvaline were thus prepared in 83-95% yield in one step from simple substrates.
- Guérard-Hélaine, Christine,Heuson, Egon,Ndiaye, Moussa,Gourbeyre, Léa,Lemaire, Marielle,Hélaine, Virgil,Charmantray, Franck,Petit, Jean-Louis,Salanoubat, Marcel,De Berardinis, Véronique,Gefflaut, Thierry
-
supporting information
p. 5465 - 5468
(2017/07/06)
-
- Process for producing an optically active gamma-hydroxy-L-glutamic acid
-
An object of the present invention is to provide a process for producing an optically active γ-hydroxy-L-glutamic acid advantageously on an industrial scale. The present invention provides a process for producing an optically active γ-hydroxy-L-glutamic acid, which comprises allowing biocatalyst I, an amino group donor, pyruvic acid and glyoxylic acid to coexist in an aqueous medium to form the optically active γ-hydroxy-L-glutamic acid in the aqueous medium, and collecting the formed optically active γ-hydroxy-L-glutamic acid therefrom, said biocatalyst I having activity of forming the optically active γ-hydroxy-L-glutamic acid from pyruvic acid and glyoxylic acid in the presence of an amino group donor. The present invention also provides a process for producing an optically active γ-hydroxy-L-glutamic acid, which comprises allowing biocatalyst II, an amino group donor and optically active 4-hydroxy-2-ketoglutaric acid to coexist in an aqueous medium to form the optically active γ-hydroxy-L-glutamic acid in the aqueous medium, and collecting the formed optically active 4-hydroxy-2-ketoglutaric acid therefrom, said biocatalyst II having activity of converting an optically active 4-hydroxy-2-ketoglutaric acid into the optically active γ-hydroxy-L-glutamic acid in the presence of an amino group donor.
- -
-
-
