132-06-9Relevant articles and documents
Multi-level logic gate operation based on amplified aptasensor performance
Feng, Lingyan,Lyu, Zhaozi,Offenh?usser, Andreas,Mayer, Dirk
, p. 7693 - 7697 (2015)
Conventional electronic circuits can perform multi-level logic operations; however, this capability is rarely realized by biological logic gates. In addition, the question of how to close the gap between biomolecular computation and silicon-based electrical circuitry is still a key issue in the bioelectronics field. Here we explore a novel split aptamer-based multi-level logic gate built from INHIBIT and AND gates that performs a net XOR analysis, with electrochemical signal as output. Based on the aptamer-target interaction and a novel concept of electrochemical rectification, a relayed charge transfer occurs upon target binding between aptamer-linked redox probes and solution-phase probes, which amplifies the sensor signal and facilitates a straightforward and reliable diagnosis. This work reveals a new route for the design of bioelectronic logic circuits that can realize multi-level logic operation, which has the potential to simplify an otherwise complex diagnosis to a "yes" or "no" decision. Yes or no? A novel split aptamer-based multi-level logic gate, which is built from INHIBIT and AND gates, performs a net XOR analysis, with an electrochemical signal as output. This work reveals a new route for the design of bioelectronic logic circuits that can realize multi-level logic operations and which simplify an otherwise complex diagnosis to a yes/no decision.
Highly regioselective methylation of inosine nucleotide: an efficient synthesis of 7-methylinosine nucleotide
Senthilvelan, Annamalai,Shanmugasundaram, Muthian,Kore, Anilkumar R.
, p. 1011 - 1019 (2020/03/30)
A facile, straightforward, reliable, and an efficient chemical synthesis of inosine nucleotides such as 7-methylinosine 5′-O-monophosphate, 7-methylinosine 5′-O-diphosphate, and 7-methylinosine 5′-O-triphosphate, starting from the corresponding inosine nucleotide is delineated. The present methylation reaction of inosine nucleotide utilizes dimethyl sulfate as a methylating agent and water as a solvent at room temperature. It is noteworthy that the present methylation reaction proceeds smoothly under aqueous conditions that is highly regioselective to afford exclusive 7-methylinosine nucleotide in good yields with high purity (>99.5%).
