Five-component cascade synthesis of nucleotide analogues in an engineered self-immobilized enzyme aggregate
(Chemical Equation Presented) Pathway in a particle: A five-enzyme biosynthetic pathway was self-immobilized to form a single biocatalyst for generation of purine nucleotide analogues from d-ribose. This method provides an alternative to engineering biosynthetic pathways in whole cells that obviates problems associated with toxicity, transport and genetic regulation.