135795-62-9

Basic information

• Product Name: NHS-FLUORESCEIN
• Synonyms: NHS-FLUORESCEIN
• CAS NO: 135795-62-9
• Molecular Formula: C₂₄H₁₅NO₇
• Molecular Weight: 429.38
• Mol File: 135795-62-9.mol

Chemical Properties

• Boiling Point: 720.6±70.0 °C(Predicted)
• Appearance: /
• Density: 1.58±0.1 g/cm3(Predicted)
• PKA: 9.05±0.20(Predicted)
• CAS DataBase Reference: NHS-FLUORESCEIN(CAS DataBase Reference)
• NIST Chemistry Reference: NHS-FLUORESCEIN(135795-62-9)
• EPA Substance Registry System: NHS-FLUORESCEIN(135795-62-9)

Safety Data

• Hazardous Substances Data: 135795-62-9(Hazardous Substances Data)

Usage

Uses

Used in Biomedical Research:
NHS-Fluorescein is used as a fluorescent labeling reagent for proteins and other biomolecules to facilitate the study of biological processes. Its ability to bind to amine groups allows researchers to easily label molecules, making it a valuable tool for visualizing and tracking their movement and localization within biological systems.
Used in Drug Development:
In the pharmaceutical industry, NHS-Fluorescein is used as a fluorescent probe to monitor drug-target interactions and to study the pharmacokinetics and biodistribution of drug candidates. Its fluorescent properties enable researchers to observe the binding, uptake, and distribution of drugs in living organisms, providing crucial information for drug optimization and development.
Used in Diagnostic Imaging:
NHS-Fluorescein is employed as a contrast agent in various diagnostic imaging techniques, such as fluorescence microscopy and fluorescence-activated cell sorting (FACS). Its ability to label and visualize specific molecules or cellular structures allows for the detection and analysis of various biological phenomena, including cell signaling, protein-protein interactions, and cellular processes.
Used in Biosensors and Bioassays:
In the field of biosensors and bioassays, NHS-Fluorescein is used as a fluorescent reporter to detect and quantify specific biomolecules or environmental factors. Its reactivity with amine groups allows for the development of highly sensitive and selective assays for the detection of target molecules, such as enzymes, antibodies, or small molecules.
Used in Tissue Engineering and Regenerative Medicine:
NHS-Fluorescein is utilized in tissue engineering and regenerative medicine as a fluorescent marker to study the behavior of cells within engineered tissues or scaffolds. Its ability to label cells and biomolecules enables researchers to monitor cell migration, proliferation, and differentiation, as well as the integration of engineered tissues with the host organism.

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 2321-07-5 fluorescein 
• 518-45-6 fluorescein free acid 

Downstream Products

• 1446245-17-5 4-(4-(2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl)piperazin-1-yl)-4-oxo-N-phenylbutanamide 
• 1415730-00-5 N-[4-(1,3,2-dithiarsolan-2-yl)phenyl]-4-{4-[2-(6-hydroxy-3-oxo-3H-xan-then-9-yl)benzoyl]piperazin-1-yl}-4-oxobutanamide 
• 1355342-72-1 C₂₉H₃₀N₂O₆ 
• 442151-48-6 [9-(2-dimethylcarbamoylphenyl)-6-oxo-6H-xanthen-3-yloxy]acetic acid methyl ester 
• 442151-46-4 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-N,N-dimethylbenzamide 

Relevant articles and documents

An in-situ detection of biological in vivo adjacent mercat protein compound and its method of

The invention belongs to the technical field of biological detection, and specifically relates to a compound and a method for in-situ detecting o-sulfhydryl protein in an organism. A kind of micromolecular fluorescent probes capable of specifically detecting o-sulfhydryl of protein are designed and synthesized, and a method for in-situ fluorescence labeling and detection of endogenous o-sulfhydryl protein in the organism is built by combining fluorescence polarization technology, protein fluorescent electrophoresis and confocal fluorescence microtechnique.

Versatile probes for the selective detection of vicinal-dithiol-containing proteins: Design, synthesis, and application in living cells

Endogenous vicinal-dithiol-containing proteins (VDPs) that have two thiol groups close to each other in space play a significant importance in maintaining the cellular redox microenvironment. Approaches to identify VDPs mainly rely on monitoring the different concentration of monothiol and total thiol groups or on indirect labeling of vicinal thiols by using p-aminophenylarsenoxide (PAO). Our previous work has reported the direct labeling of VDPs with a highly selective receptor PAO analogue, which could realize fluorescence detection of VDPs directly in living cells. Herein, we developed a conjugated approach to expand detectable tags to nitrobenzoxadiazole (NBD), fluorescein, naphthalimide, and biotin for the synthesis of a series of probes. Different linkers have also been introduced toward conjugation of VTA2 with these functional tags. These synthesized flexible probes with various features will offer new tools for the potential identification and visualization of vicinal dithiols existing in different regions of VDPs in living cells. These probes are convenient tools for proteomics studies of various disease-related VDPs and for the discovery of new drug targets. Copyright

A simple FRET-based modular design for diagnostic probes

In recent years, there has been a massive effort to develop molecular probes with optical modes of action. Probes generally produce detectable signals based on changes in fluorescence properties. Here, we demonstrate the potential of self-immolative molecular adaptors as a platform for Turn-On probes based on the FRET technique. The probe is equipped with identical fluorophore pairs or a fluorophore/quencher FRET pair and a triggering substrate. Upon reaction of the analyte of interest with the triggering substrate, the self-immolative adaptor spontaneously releases the two dye molecules to break off the FRET effect. As a result, a new measurable fluorescent signal is generated. The fluorescence obtained can be used to quantify the analyte. The modular structure of the probe design will allow the preparation of various chemical probes based on the FRET activation technique.

Xanthamide fluorescent dyes

Two derivatives of fluorescein, termed "xanthamides," were prepared from fluorescein, an inexpensive dye. Relative to fluorescein, which contains a 6-phenolic OH and a 2′-carboxyl, the first derivative (5) contains a carboxymethyl ether at the 6-position