1363376-98-0

Basic information

• Product Name: DTBA
• Synonyms: 2,4-Dihydroxy-3,5,6-trimethylbenzoic acid;DTBA;USF-406B;(2S)-2-Amino-1,4-dimercaptobutane hydrochloride;(S)-2-Aminobutane-1,4-dithiol hydrochloride;(S)-2-Aminobutane-1,4-dithiol hydrochloride 99% (titration)
• CAS NO: 1363376-98-0
• Molecular Formula: C4H12ClNS2
• Molecular Weight: 173.72778
• Mol File: 1363376-98-0.mol

Chemical Properties

• Melting Point: 210-225°C
• Appearance: /
• Storage Temp.: 2-8°C
• CAS DataBase Reference: DTBA(CAS DataBase Reference)
• NIST Chemistry Reference: DTBA(1363376-98-0)
• EPA Substance Registry System: DTBA(1363376-98-0)

Safety Data

• Hazard Codes: Xn
• Statements: 22-36/37/38
• Safety Statements: 26
• WGK Germany: 3
• Hazardous Substances Data: 1363376-98-0(Hazardous Substances Data)

Usage

Uses

Used in Biochemical Research:
DTBA is used as a reducing agent for [application reason] faster reduction of small molecule disulfides and activation of cysteine-dependent proteases compared to other reagents like DTT. This makes it a valuable tool in biochemical research for studying protein function and stability.
Used in Pharmaceutical Industry:
DTBA is used as a reagent for [application reason] its ability to enhance the reduction of disulfide bonds in drug molecules, which can improve the efficacy and stability of certain pharmaceutical compounds.
Used in Protein Engineering:
DTBA is used as a reducing agent for [application reason] its faster reduction capabilities, which can be beneficial in protein engineering applications where the modification of disulfide bonds is crucial for protein function and structure.
Used in Enzyme Activation:
DTBA is used as an activator for [application reason] its ability to rapidly activate cysteine-dependent proteases, such as papain, which can be important in various biotechnological and industrial processes.

Relevant articles and documents

DITHIOAMINE REDUCING AGENTS

Dithioamine reducing agents useful for the reduction of disulfide bonds. The reducing agents of this invention are useful, for example, to reduce disulfide bonds, particularly in proteins, or to prevent the formation of disulfide bonds, particularly in proteins and other biological molecules. Reducing agents of this invention can be employed to regulate protein function in proteins in which a sulfhydryl group is associated with biological activity. Reducing agents of this invention can prevent inactivation of a given protein or enhance activation of a given protein or other biological molecule in vitro and/or in vivo. Reducing agents of this invention can prevent or reduce oxidation of cysteine residues in proteins and prevent the formation of reduced activity protein dimers (or other oligomers). Reducing agents of this invention are useful and suitable for application in a variety of biological applications, particularly as research and synthetic reagents.

A potent, versatile disulfide-reducing agent from aspartic acid

Dithiothreitol (DTT) is the standard reagent for reducing disulfide bonds between and within biological molecules. At neutral pH, however, >99% of DTT thiol groups are protonated and thus unreactive. Herein, we report on (2S)-2-amino-1,4-dimercaptobutane (dithiobutylamine or DTBA), a dithiol that can be synthesized from l-aspartic acid in a few high-yielding steps that are amenable to a large-scale process. DTBA has thiol pKa values that are ～1 unit lower than those of DTT and forms a disulfide with a similar E o′ value. DTBA reduces disulfide bonds in both small molecules and proteins faster than does DTT. The amino group of DTBA enables its isolation by cation-exchange and facilitates its conjugation. These attributes indicate that DTBA is a superior reagent for reducing disulfide bonds in aqueous solution.