- A novel two-photon fluorescent probe for hydrogen sulfide in living cells using an acedan-NBD amine dyad based on FRET process with high selectivity and sensitivity
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Two-photon imaging, due to its unique advantage to utilize near-infrared light as the excitation source, has emerged as an important tool for biomedical research. Herein, the first NBD amine based two-photon fluorescence probe L using a FRET strategy was developed for detecting H2S in vitro and in vivo. The probe L not only afforded high selectivity and sensitivity for the detection of H2S, but also displayed a linear response to H2S with a low detection limit of 24 nM. Furthermore, the living cell experiments indicated that L can be effective for the both exogenous and endogenous detection of H2S inside living cells by two-photon fluorescence imaging.
- Tang, Yao,Jiang, Guo-Fang
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- Platinum(II) as bifunctional linker in antibody-drug conjugate formation: Coupling of a 4-nitrobenzo-2-oxa-1,3-diazole fluorophore to trastuzumab as a model
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The potential of platinum(II) as a bifunctional linker in the coordination of small molecules, such as imaging agents or (cytotoxic) drugs, to monoclonal antibodies (mAbs) was investigated with a 4-nitrobenzo-2-oxa-1,3-diazole (NBD) fluorophore and trastu
- Waalboer, Dennis C. J.,Muns, Joey A.,Sijbrandi, Niels J.,Schasfoort, Richard B. M.,Haselberg, Rob,Somsen, Govert W.,Houthoff, Hendrik-Jan,Van Dongen, Guus A. M. S.
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- A GLUT1 inhibitor-based probe significantly ameliorates the sensitivity of tumor detection and diagnostic imaging
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We report a non-antibody GLUT1 inhibitor probe NBDQ that is 30 times more sensitive than the traditional GLUT1 transportable tracer for cancer cell imaging and Warburg effect-based tumor detection. NBDQ reveals significant advantages in terms of tumor sel
- Liu, Shengnan,Song, Weijie,Cui, Yujun,Sun, Ziru,Huang, Zhenhua,Gao, Qingzhi
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- 4-Nitro-7-piperazino-2,1,3-benzoxadiazole as a reagent for monitoring of airborne isocyanates by liquid chromatography
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4-Nitro-7-piperazino-2,1,3-benzoxadiazole (NBDPZ) is presented as a new reagent for the determination of mono- and diisocyanates in air samples. NBDPZ readily reacts with the airborne analytes, thus yielding the corresponding urea derivatives, which are s
- Vogel, Martin,Karst, Uwe
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- Investigation of thiolysis of NBD amines for the development of H2S probes and evaluating the stability of NBD dyes
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In order to evaluate the thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) amines for development of H2S probes, herein we investigated the reactivity and selectivity of a series of NBD amines for the first time. The piperazinyl- and piperidyl-ba
- Song, Fanbo,Li, Zhifei,Li, Jiayuan,Wu, Shuai,Qiu, Xianbo,Xi, Zhen,Yi, Long
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- Synthesis and characterizations of a highly sensitive and selective fluorescent probe for hydrogen sulfide
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Hydrogen sulfide (H2S) is an important endogenous signaling molecule with a variety of biological functions. To detect H2S in living biological systems, herein we developed a new fluorescent probe for highly sensitive and selective s
- Huang, Yan,Zhang, Changyu,Xi, Zhen,Yi, Long
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- Triphenylamine derivative, preparation method thereof and application of triphenylamine derivative in double-channel fluorescence detection of hydrogen sulfide
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The invention belongs to the technical field of fluorescent probes for H2S detection, and provides a triphenylamine derivative, a preparation method thereof, and application of the triphenylamine derivative in double-channel fluorescence detection of hydr
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- 7-nitro-1,2,3-benzooxadiazole derivative and synthesis method and application thereof
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The invention provides a 7-nitro-1,2,3-benzoxadiazole (NBD) derivative and a synthesis method and application thereof, and belongs to the field of detection of NBD derivatives and mercury ions. The synthesis method of the NBD derivatives comprises the following steps of: first, dissolving 4-chlorine-7-nitrobenzo-1,2,3-benzoxadiazole and anhydrous piperazine in 2-methoxyethanol, heating and refluxing, and separating by column chromatography to obtain a compound 1; then, heating and refluxing 2,6-bis (chloromethyl) pyridine and 8-hydroxyquinoline in an acetone solution, and separating by the column chromatography to obtain a compound 2; finally, dissolving the compound 1 and the compound 2 in acetonitrile, refluxing for 12 hours, and separating by the column chromatography to obtain the product NBD derivative. The invention also provides a method for detecting the mercury ions, which comprises the step of quantitatively detecting the content of Hg in a solution of all water (pH = 7.4) by using the NBD derivative as a probe. The detection method can detect the mercury ions in an aqueous solution with high selectivity and sensitivity, and is simple, convenient and rapid to operate.Meanwhile, test paper for detecting the mercury ions is provided, so that the Hg detection is more convenient.
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Paragraph 0014; 0036; 0037
(2019/06/07)
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- A highly selective and sensitive fluorescent probe for simultaneously distinguishing and sequentially detecting H2S and various thiol species in solution and in live cells
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A novel dual-channel fluorescent probe (NCR) based on differences in reactivity among H2S, Cys/Hcy, and GSH was rationally designed for simultaneously distinguishing and sequentially sensing H2S, Cys/Hcy, and GSH using two emission channels, which also demonstrated that NCR can be used for targeting mitochondria in mammalian cells.
- Qiao, Dan,Shen, Tangliang,Zhu, Mengyuan,Liang, Xiao,Zhang, Lu,Yin, Zheng,Wang, Binghe,Shang, Luqing
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supporting information
p. 13252 - 13255
(2018/12/11)
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- Benzooxaoxadiazole derivative as well as preparation method and application thereof
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The invention discloses a benzooxaoxadiazole derivative as well as a preparation method and application thereof. The benzooxaoxadiazole derivative is prepared as follows: firstly, NBD-PZ is obtained by reaction between NBD-Cl and piperazine; then 4-nitro-7-(N-1'-acryloylpiperazine)-2,1,3-benzoxoxaoxadiazole is obtained by reaction between NBD-PZ and acryloyl chloride. The benzooxaoxadiazole derivative BGAS disclosed by the invention as a fluorescent probe has high selectivity for cysteine, can specifically detect the cysteine, particularly has a rapid detection effect on the cysteine in waterand simultaneously has relatively high sensitivity, thereby being applied to the detection of the cysteine in living cells.
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Paragraph 0025; 0026; 0027
(2018/09/21)
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- NBD-organic amine fluorescence probe, and preparation method and application thereof
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The invention discloses an NBD-organic amine fluorescence probe, and a preparation method and an application thereof. The structure of the fluorescence probe is represented by formula (1); and in the formula (1), m is 0 or an integer in a range of 1 to 5,
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- Dual-Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2S
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Hydrogen sulfide (H2S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H2S is necessary. We show here that dual-reactable fluorescent H2S probes could react with higher selectivity than single-reactable probes. One of the dual-reactable probes gives more than 4000-fold turn-on response when reacting with H2S, the largest response among fluorescent H2S probes reported thus far. In addition, the probe could be used for high-throughput enzymatic assays and for the detection of Cys-induced H2S in cells and in zebrafish. These dual-reactable probes hold potential for highly selective and sensitive detection of H2S in biological systems. Two heads are better than one: Fluorescent probes with two types of reactive heads (R1 and R2) for H2S can lead to a higher selectivity than that of single-reactable probes (See Figure). The probe was used for highly selective and sensitive detection of biological H2S.
- Wei, Chao,Wang, Runyu,Zhang, Changyu,Xu, Guoce,Li, Yanyan,Zhang, Qiang-Zhe,Li, Lu-Yuan,Yi, Long,Xi, Zhen
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p. 1376 - 1381
(2016/05/19)
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- A fluorescent turn-on H2S-responsive probe: Design, synthesis and application
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Hydrogen sulfide (H2S) is considered as the third signaling molecule in vivo and it plays an important role in various physiological processes and pathological processes in vivo, such as vasodilation, apoptosis, neurotransmission, ischemia/reperfusion-induced injury, insulin secretion and inflammation. Developing a highly selective and sensitive method that can detect H2S in the biological system is very important. In this work, a colorimetric and "turn-on" fluorescent probe is developed. Furthermore, this probe displays a highly selective response to H2S in aqueous solution and possesses good capability for bioimaging H2S without interference in living cells. The results suggest that a H2S-selective probe has good water-solubility, biocompatibility and cell-penetrability and can serve as an efficient tool for probing H2S in the cell level.
- Zhang, Yufeng,Chen, Haiyan,Chen, Dan,Wu, Di,Chen, Xiaoqiang,Liu, Sheng Hua,Yin, Jun
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p. 9760 - 9776
(2015/10/05)
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- Synthesis of conjugated spermine derivatives with 7-nitrobenzoxadiazole (NBD), rhodamine and bodipy as new fluorescent probes for the polyamine transport system
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The synthesis of a series of conjugated spermine derivatives with benzoxadiazole, phenylxanthene or bodipy fluorophores is described. These fluorescent probes were used to identify the activity of the polyamine transport system (PTS). N1-Methylspermine NBD conjugate 5 proved to have the optimal fluorescence characteristics and was used to show a selectivity for PTS-proficient CHO versus PTS-deficient CHO-MG cells. It can therefore be used as a tool for the selection of cells sensitive to cytotoxic compounds vectored through the PTS.
- Guminski, Yves,Grousseaud, Martial,Cugnasse, Sandrine,Brel, Viviane,Annereau, Jean-Philippe,Vispe, Stephane,Guilbaud, Nicolas,Barret, Jean-Marc,Bailly, Christian,Imbert, Thierry
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scheme or table
p. 2474 - 2477
(2009/12/25)
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- High yield selective acylation of polyamines: Proton as protecting group
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An advance in the selective acylation of polyamines having identical or similar amine functions is reported. While nucleophilicity differences between the various amine functions are slight, the corresponding conjugate acids exhibit pKa values
- Oganesyan, Asmik,Cruz, Iris A.,Amador, Roberto B.,Sorto, Nohemy A.,Lozano, Jose,Godinez, Carlos E.,Anguiano, Jaime,Pace, Heather,Sabih, Ghiwa,Gutierrez, Carlos G.
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p. 4967 - 4970
(2008/03/28)
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- Ubiquitin ligase inhibitors
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This invention describes compounds and pharmaceutical compositions useful as ubiquitin agent inhibitors, particularly ubiquitin ligase inhibitors. The compounds and pharmaceutical compositions of the invention are useful as inhibitors of the biochemical p
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Page/Page column 23
(2008/06/13)
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- Modular fluorescent-labeled siderophore analogues
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Biomimetic analogues 1 of the microbial siderophore (iron carrier) ferrichrome were labeled via piperazine with various fluorescent markers at a site not interfering with iron binding or receptor recognition (compounds 10- 12). These iron carriers were bu
- Nudelman, Raphael,Ardon, Orly,Hadar, Yitzhak,Chen, Yona,Libman, Jacqueline,Shanzer, Abraham
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p. 1671 - 1678
(2007/10/03)
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