- Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics
-
The inverse electron demand Diels–Alder pyridazine elimination reaction between tetrazines and allylic substituted trans-cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylamine substrates has so far proven difficult. Here, we report a fluorogenic tool consisting of a TCO-linked EDANS fluorophore and a DABCYL quencher for accurate determination of both the click and release rate constants for any tetrazine at physiologically relevant concentrations.
- de Geus, Mark A. R.,Maurits, Elmer,Sarris, Alexi J. C.,Hansen, Thomas,Kloet, Max S.,Kamphorst, Kiki,ten Hoeve, Wolter,Robillard, Marc S.,Pannwitz, Andrea,Bonnet, Sylvestre A.,Codée, Jeroen D. C.,Filippov, Dmitri V.,Overkleeft, Herman S.,van Kasteren, Sander I.
-
supporting information
p. 9900 - 9904
(2020/06/05)
-
- ENZYME DETECTION COMPOUND AND MANUFACTURING METHOD OF THE COMPOUND
-
PROBLEM TO BE SOLVED: To obtain a noel compound for detecting enzyme and a detection method of enzyme using the same and a compound having following structure, R1 and R2 are each independently H, an alkyl group having 1 to 2 carbon atoms, an alkenyl group having 2 carbon atoms or an alkynyl group or a phenyl group having 2 carbon atoms, n is 2 to 12 and X is an amino acid residue. SELECTED DRAWING: Figure 2 COPYRIGHT: (C)2018,JPOandINPIT
- -
-
Paragraph 0039
(2018/02/27)
-
- TUNABLE FLUORESCENCE USING CLEAVABLE LINKERS
-
The invention relates to cleavable chemistry in general, and in particular, to tunable fluoresence using cleavable linkers present in fluorochrome-quencher conjugates.
- -
-
Paragraph 0070-0071
(2014/11/11)
-
- Multicolor, one- and two-photon imaging of enzymatic activities in live cells with fluorescently quenched activity-based probes (qABPs)
-
Fluorescence imaging provides an indispensable way to locate and monitor biological targets within complex and dynamic intracellular environments. Of the various imaging agents currently available, small molecule-based probes provide a powerful tool for live cell imaging, primarily due to their desirable properties, including cell permeability (as a result of their smaller sizes), chemical tractability (e.g., different molecular structures/designs can be installed), and amenability to imaging a wide variety of biological events. With a few exceptions, most existing small molecule probes are however not suitable for in vivo bioimaging experiments in which high-resolution studies of enzyme activity and localization are necessary. In this article, we reported a new class of fluorescently Quenched Activity-Based Probes (qABPs) which are highly modular, and can sensitively image (through multiple enzyme turnovers leading to fluorescence signal amplification) different types of enzyme activities in live mammalian cells with good spatial and temporal resolution. We have also incorporated two-photon dyes into our modular probe design, enabling for the first time activity-based, fluorogenic two-photon imaging of enzyme activities. This, hence, expands the repertoire of smart, responsive probes currently available for live cell bioimaging experiments.
- Hu, Mingyu,Li, Lin,Wu, Hao,Su, Ying,Yang, Peng-Yu,Uttamchandani, Mahesh,Xu, Qing-Hua,Yao, Shao Q.
-
supporting information; experimental part
p. 12009 - 12020
(2011/09/21)
-
- Synthesis of dye-labeled lysine derivatives
-
The N(ε)-dabcyl-N(α)-(9-fluorenylmethoxy)-carbonyllysine was prepared by reaction of lysine-Cu2+ complex with the N-hydroxysuccinimide (HOSu) activated ester of [4-(4'-dimethylamino)phenylazo]benzoic acid (dabcyl acid) followed by treatment with EDTA and acylation with Fmoc-OSu, and the N(α)tert-butyloxycarbonyl-N(ε)-dabcyllysine was prepared by reaction of N(α)-tertbutyloxycarbonyllysine with dabcyl-OSu.
- Shen, Zongxuan,Zhang, Yawen,Chen, Yan
-
p. 2525 - 2532
(2007/10/03)
-