17833-43-1 Usage
Uses
Used in Enzyme Assays:
4-Methylumbelliferyl-α-D-glucopyranoside hydrate is used as a fluorogenic substrate for the identification, characterization, and kinetic analysis of α-glucosidase enzymes. Its fluorescence properties allow for the quantification of enzyme activity and have been utilized in the study of Pompe disease.
Used in Pompe Disease Research:
In the field of medical research, 4-Methylumbelliferyl-α-D-glucopyranoside hydrate is used as a fluorogenic substrate in the assay of α-glucosidase, specifically for studying Pompe disease. This lysosomal storage disorder is characterized by a deficiency in the enzyme α-glucosidase, and the substrate helps in understanding the disease progression and evaluating potential treatments.
Used in Fabry Disease Biomarker Detection:
4-Methylumbelliferyl-α-D-glucopyranoside hydrate has been employed to quantify α-glucosidase activity in infant blood spot samples as a biomarker for Fabry and Pompe diseases. These lysosomal storage disorders are identified by enzyme deficiencies, and the substrate aids in the early detection and diagnosis of these conditions.
Purification Methods
Recrystallise 4-methylumbellifer-7-yl--D-glucopyranoside from hot H2O or EtOH. [Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 IV 443.]
Check Digit Verification of cas no
The CAS Registry Mumber 17833-43-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,7,8,3 and 3 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 17833-43:
(7*1)+(6*7)+(5*8)+(4*3)+(3*3)+(2*4)+(1*3)=121
121 % 10 = 1
So 17833-43-1 is a valid CAS Registry Number.
InChI:InChI=1/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m0/s1
17833-43-1Relevant articles and documents
Concise synthesis of sulfoquinovose and sulfoquinovosyl diacylglycerides, and development of a fluorogenic substrate for sulfoquinovosidases
Zhang, Yunyang,Mui, Janice W.-Y.,Arumaperuma, Thimali,Lingford, James P.,Goddard-Borger, Ethan D.,White, Jonathan M.,Williams, Spencer J.
, p. 675 - 686 (2020)
The sulfolipid sulfoquinovosyl diacylglycerol (SQDG) and its headgroup, the sulfosugar sulfoquinovose (SQ), are estimated to harbour up to half of all organosulfur in the biosphere. SQ is liberated from SQDG and related glycosides by the action of sulfoquinovosidases (SQases). We report a 10-step synthesis of SQDG that we apply to the preparation of saturated and unsaturated lipoforms. We also report an expeditious synthesis of SQ and (13C6)SQ, and X-ray crystal structures of sodium and potassium salts of SQ. Finally, we report the synthesis of a fluorogenic SQase substrate, methylumbelliferyl α-d-sulfoquinovoside, and examination of its cleavage kinetics by two recombinant SQases. These compounds will assist in dissecting the role of sulfoglycolysis in the biogeochemical sulfur cycle and understanding the molecular basis of sulfoglycolysis.
