- Use of a 13C atom to differentiate two 15N-labeled nucleosides
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We report the first examples of the specifically 15N and 13C multilabeled nucleosides: [1,NH2-15N2]- and [2-13C-1,NH2-15N2-]- guanosine; [1,7,NH2-15N3]- and [2-13C-1,7,NH2-15N3]-2'- deoxyguanosine. In each set, the [13C] atom functions as a 'tag' that allows the N1 and N2 15N atoms of two 15N-labeled guanines to be unambiguously differentiated in RNA and DNA fragments. The syntheses employ high-yield reactions in which protecting groups are not required and use relatively low cost sources of isotopes: [15N]-ammonium chloride and [15N]- or [13C,15N]-potassium cyanide.
- Zhao,Pagano,Wang,Shallop,Gaffney,Jones
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p. 7832 - 7835
(2007/10/03)
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