209734-74-7

Basic information

• Product Name: 5-ROX, SE
• Synonyms: 5-ROX, SE;5(6)-ROX, SE;5-(AND-6)-CARBOXY-X-RHODAMINE, SUCCINIMIDYL ESTER;5-CARBOXY-X-RHODAMINE N-SUCCINIMIDYL ESTER;5-CARBOXY-X-RHODAMINE, SUCCINIMIDYL ESTER;5-Carboxy-X-rhodamin N-succinimidyl ester;5-ROX SE, 5-CXR SE;5-Carboxy-X-rhodamine NHS ester
• CAS NO: 209734-74-7
• Molecular Formula: C₃₇H₃₃N₃O₇
• Molecular Weight: 631.68
• Product Categories: Biochemicals and Reagents;DetectionFluorescent Stains;Fluorescent Labels;Fluorescent Probes, Labels, Particles and Stains;Nucleic Acid Stains;Nucleic Acid Stains For MicroscopyFluorescent Probes, Labels, Particles and Stains;Rhodamine and Derivatives
• Mol File: 209734-74-7.mol

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• Solubility: DMF: soluble
• CAS DataBase Reference: 5-ROX, SE(CAS DataBase Reference)
• NIST Chemistry Reference: 5-ROX, SE(209734-74-7)
• EPA Substance Registry System: 5-ROX, SE(209734-74-7)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 209734-74-7(Hazardous Substances Data)

Usage

Uses

Used in Molecular Biology:
5-ROX, SE is used as a labeling reagent for the preparation of charge-modified dye-labeled ddNTPs for "direct-load" DNA sequencing. Its fluorescence properties allow for the efficient detection and analysis of DNA sequences, making it a valuable tool in molecular biology research.
Used in Diagnostics:
In the field of diagnostics, 5-ROX, SE is utilized as a fluorescent marker in various assays and tests. Its ability to bind specifically to target molecules makes it a useful tool for detecting and identifying specific biological entities, such as pathogens or genetic mutations.
Used in Drug Discovery:
5-ROX, SE is also employed in drug discovery processes, where it can be used to label and track the interactions between potential drug candidates and their target molecules. This helps researchers understand the mechanisms of action and optimize the development of new therapeutic agents.
Used in Biotechnology:
In the biotechnology industry, 5-ROX, SE is used in various applications, such as the development of biosensors and the study of protein-protein interactions. Its fluorescence properties enable the monitoring of biological processes and the detection of specific molecular interactions in real-time.
Used in Research and Development:
5-ROX, SE is a valuable tool in research and development, particularly in the fields of genomics, proteomics, and cell biology. Its ability to label and visualize specific molecules allows researchers to gain insights into the complex interactions and processes occurring within biological systems.

Upstream product

• 216699-35-3 5-carboxy-X-rhodamine 
• 105832-38-0 O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate 

Downstream Products

• 960215-37-6 1-(5-carboxy-X-rhodaminyl)-4-(4-methylbenzoyl)piperazine 
• 1537876-09-7 N-{(5-carboxy-X-rhodaminyl)but-4-yl}-2-{1-(4-chlorobenzoyl)-5-methoxy-2′-trifluoromethyl-1H-indol-3-yl}-acetamide 

Relevant articles and documents

Targeted Detection of Cyclooxygenase-1 in Ovarian Cancer

Overexpression of cyclooxygenase-1 (COX-1) is associated with the initiation and progression of ovarian cancer, and targeted imaging of COX-1 is a promising strategy for early detection of this disease. We report the discovery of N-[(5-carboxy-X-rhodaminy

Synthesis of 5- and 6-carboxy-x-rhodamines

(Equation Presented) An efficient route is reported to 5- and 6-carboxy-X-rhodamines (compounds 1 and 2) that contain multiple n-propylene or y,y-dimethylpropylene groups bridging terminal nitrogen atoms and the central xanthene core. Gram quantities of these dyes are synthesized from inexpensive starting materials. The isolated products are activated by selective transformation of the carboxylic acid group into N-hydroxysuccinimidyl esters in situ and then conjugated with an amino group of a molecule of interest.