- Improved selective protections of L-arginine
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Reliable procedures for the selective introduction of carbobenzyloxy and phthalimido groups onto the α-amino position, and of carboethoxy groups onto the δ- and ω-guanidino positions of L-arginine are described.
- Moynihan, Humphrey A.,Yu, Weiping
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- Isolation and characterization of Nα-Benzyloxycarbonyl amino Acid Urethane Hydrolase II from Lactobacillus fermenti 36 ATCC 9338
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A novel enzyme, which was named Nα-benzloxycarbonyl amino acid urethane hydrolase II, was purified from a cell-free extract of Lactobacillus fermenti 36ATCC 9338.The enzyme catalyzed the stoichiometric hydrplysis of N-α-benzyloxycarbonyl arginine to form benzyl alcohol and arginine.The enzyme was purified 106-fold with an activity yield of 3percent.The purified enzyme was homogeneous by disc gel electrophoresis.The molecular weight of native enzyme is about 200,000 by gel filtration, and a molecular weight of 27,000 was found for the reduced and denaturated enzyme was 5.0, it was inhibited by disodium ethylenediamine tetraacetate and p-chloromercuribenzoate, and the presence of a divalent cation, i.e.Co2+, is essential for its activity.
- Matsamura, Eiko,Yamamoto, Eiko,Kawano, Tatsu,Shin, Takashi,Murao, Sawao
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p. 1563 - 1572
(2007/10/02)
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