27999-55-9 Usage
Uses
Used in Pharmaceutical Industry:
5'-Tosyl-2'-deoxy Cytidine is used as a key intermediate for the synthesis of vitamin B12 coenzyme analogs containing 2'-deoxynucleoside. These analogs are essential in various pharmaceutical applications, particularly in the development of drugs targeting specific metabolic pathways and enzymatic processes.
Used in Chemical Synthesis:
As a nucleoside, 5'-Tosyl-2'-deoxy Cytidine is utilized in the chemical synthesis of various compounds, including those with potential therapeutic applications. Its unique structure allows for the creation of novel molecules with specific biological activities, contributing to the advancement of drug discovery and development.
Used in Research and Development:
5'-Tosyl-2'-deoxy Cytidine serves as an important research tool in the field of biochemistry and molecular biology. It is used to study the structure and function of nucleic acids, as well as to investigate the mechanisms of various biological processes involving nucleosides and their derivatives.
Check Digit Verification of cas no
The CAS Registry Mumber 27999-55-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,9,9 and 9 respectively; the second part has 2 digits, 5 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 27999-55:
(7*2)+(6*7)+(5*9)+(4*9)+(3*9)+(2*5)+(1*5)=179
179 % 10 = 9
So 27999-55-9 is a valid CAS Registry Number.
InChI:InChI=1/C16H19N3O6S/c1-10-2-4-11(5-3-10)26(22,23)24-9-13-12(20)8-15(25-13)19-7-6-14(17)18-16(19)21/h2-7,12-13,15,20H,8-9H2,1H3,(H2,17,18,21)/t12-,13-,15-/m1/s1
27999-55-9Relevant articles and documents
α,β-Methylene-2′-deoxynucleoside 5′-triphosphates as noncleavable substrates for DNA polymerases: Isolation, characterization, and stability studies of novel 2′-deoxycyclonucleosides, 3,5′-cyclo-dG, and 2,5′-cyclo-dT
Liang, Fengting,Jain, Nidhi,Hutchens, Troy,Shock, David D.,Beard, William A.,Wilson, Samuel H.,Chiarelli, M. Paul,Cho, Bongsup P.
experimental part, p. 6460 - 6470 (2009/10/23)
We report synthesis and characterization of a complete set of α,β-methylene-2′-dNTPs (α,β-m-dNTP; N = A, C, T, G, 12-15) in which the α,β-oxygen linkage of natural dNTP was replaced by a methylene group. These nucleotides were designed to be noncleavable substrates for DNA polymerases. Synthesis entails preparation of 2′-deoxynucleoside 5′-diphosphate precursors, followed by an enzymatic γ-phosphorylation. All four synthesized α,β-m-dNTPs were found to be potent inhibitors of polymerase β, with Ki values ranging 1-5 μM. During preparation of the dG and dT derivatives of α,β-methylene diphosphate, we also isolated significant amounts of 3,5′-cyclo-dG (16) and 2,5′-cyclo-dT (17), respectively. These novel 2′-deoxycyclonucleosides were formed via a base-catalyzed intramolecular cyclization (N3 → C5′ and O2 → C5′, respectively). In acidic solution, both 16 and 17 underwent glycolysis, followed by complete depurination. When exposed to alkaline conditions, 16 underwent an oxidative deamination to produce 3,5′-cyclo-2′-deoxyxanthosine (19), whereas 17 was hydrolyzed exclusively to dT.
