- In Situ Observation of mtDNA Damage during Hepatic Ischemia-Reperfusion
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Hepatic ischemia-reperfusion (IR) injury is a severe pathophysiological event during liver surgery or transplantation and could lead to liver failure or even death. The energy supply of mitochondria plays an essential role in preventing IR injury. Mitochondrial DNA (mtDNA) is involved in maintaining the balance of energy by participating in an oxidative phosphorylation process. However, the exact relationship between IR and mtDNA remains unclear by reason of the lack of an accurate real-time analysis method. Herein, we fabricated a mitochondria-targeting fluorescent probe (mtDNA-BP) to explore mtDNA stability and supervise the changes in mtDNA in IR liver. By virtue of pyridinium electropositivity and suitable size, mtDNA-BP could accumulate in mitochondria and insert into the mtDNA groove, which made mtDNA-BP fluoresce strongly. This is attributed to the reduction of the intramolecular rotation energy loss that is restricted by DNA. By in situ fluorescence imaging, we observed in real time that mtDNA damage was aggravated by deteriorating IR injury, so the ROS-mtDNA-mediated IR damage signal pathway was speculated. Furthermore, on the basis of mtDNA-BP real-time response capability for mtDNA, we established a drug-screening method for inhibiting IR injury and found superior therapeutic performance of two potential drugs: pioglitazone and salidroside. This work contributes to our understanding of mtDNA-related disease and provides a new drug analysis method.
- Zhang, Wen,Bi, Simin,Li, Ping,Liu, Jihong,Zhou, Chunmiao,Wang, Xin,Zhang, Wei,Wang, Hui,Tang, Bo
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- PROCESSES FOR PREPARING L-ALKYL-3-ALKYL-PYRIDINIUM BROMIDE AND USES THEREOF AS ADDITIVES IN ELECTROCHEMICAL CELLS
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The invention relates to the use of at least one l-alkyl-3- alkyl-pyridinium halide, in particular l-alkyl-3-methyl- pyridinium bromide, as an additive in bromine-generating electrochemical cells, such as zinc/bromine cells. Processes for preparing l-alkyl-3-methyl-pyridinium bromide and concentrated aqueous solutions comprising same for use as additives in the aforementioned cells, are also disclosed.
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Page/Page column 15; 41
(2014/08/20)
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- PROCESSES FOR PREPARING N-ETHYL-2-METHYLPYRIDINIUM BROMIDE AND N-ETHYL-3-METHYLPYRIDINIUM BROMIDE
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The invention provides processes for preparing concentrated aqueous solutions of N-ethyl-2-methylpyridinium bromide and N- ethyl-3-methylpyridinium bromide, which solutions are useful as additives in the electrolyte of hydrogen/bromine electrochemical cells. Also provided are concentrated aqueous solutions of N-ethyl-2-methylpyridinium bromide, N-ethyl-3-methylpyridinium bromide and their mixtures.
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Page/Page column 10
(2013/04/10)
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- Solid-state fluorescence of pyridinium styryl dyes
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1-Alkyl-2-[4-(diethylamino)styryl]pyridinium salts showed fluorescence maxima at around 650 nm in the solid state depending on the alkyl group and counter anion. 1-Butyl-2-[4-(diethylamino)styryl]pyridinium bis(perfluorobutylsulfonyl)imide exhibited the fluorescence maximum at 652 nm with the highest quantum yield 0.16 in the crystalline form. The single X-ray crystallography suggests that this compound exhibits isolated dimer-type packing leading to fluorescence in the solid state.
- Matsui, Masaki,Ooiwa, Kohtaro,Okada, Ayumi,Kubota, Yasuhiro,Funabiki, Kazumasa,Sato, Hiroyasu
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p. 916 - 923
(2013/09/12)
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