47729-63-5

Basic information

• Product Name: JC-1
• Synonyms: 1,1',3,3'-TETRAETHYL-5,5',6,6'-TETRACHLOROBENZIMIDAZOLOCARBOCYANINE IODIDE;1,1',3,3'-TETRAETHYL-5,5',6,6'-TETRACHLOROIMIDACARBOCYANINE IODIDE;1H-BENZIMIDAZOLIUM, 5,6-DICHLORO-2-[3-(5,6-DICHLORO-1,3-DIETHYL-1,3-DIHYDRO-2H-BENZIMIDAZOL-2-YLIDENE)-1-PROPENYL]-1,3-DIETHYL-, IODIDE;1-DODECYL-2-[(E)-3-(1-DODECYL-3,3-DIMETHYL-1,3-DIHYDRO-2H-INDOL-2-YLIDENE)-1-PROPENYL]-3,3-DIMETHYL-3H-INDOLIUM PERCHLORATE;3H-INDOLIUM, 1-DODECYL-2-[3-(1-DODECYL-1,3-DIHYDRO-3,3-DIMETHYL-2H-INDOL-2-YLIDENE)-1-PROPENYL]-3,3-DIMETHYL-, PERCHLORATE;5,5',6,6'-TETRACHLORO-1,1',3,3'-TETRAETHYLBENZIMIDAZOLYLCARBOCYANINE IODIDE;5,6-DICHLORO-2-[3-(5,6-DICHLORO-1,3-DIETHYL-2-BENZIMIDAZOLINYLIDENE)PROPENYL]-1,3-DIETHYLBENZIMIDAZOLIUM IODIDE;5,6-DICHLORO-2-[(E)-3-(5,6-DICHLORO-1,3-DIETHYL-1,3-DIHYDRO-2H-BENZIMIDAZOL-2-YLIDENE)-1-PROPENYL]-1,3-DIETHYL-3H-BENZIMIDAZOL-1-IUM IODIDE
• CAS NO: 47729-63-5
• Molecular Formula: C25H27Cl4IN4
• Molecular Weight: 652.23
• Mol File: 47729-63-5.mol

Chemical Properties

• Appearance: /solid
• Storage Temp.: -20C
• CAS DataBase Reference: JC-1(CAS DataBase Reference)
• NIST Chemistry Reference: JC-1(47729-63-5)
• EPA Substance Registry System: JC-1(47729-63-5)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 47729-63-5(Hazardous Substances Data)

Usage

Uses

Used in Cell and Tissue Analysis:
JC-1 is used as a mitochondrial potential indicator for analyzing cells and tissues. It helps in determining the mitochondrial membrane potential, which is crucial for understanding cellular processes and functions.
Used in Flow Cytometry and Fluorescent Microscopy:
JC-1 is used as a staining agent for flow cytometry and fluorescent microscopy. Its color change based on membrane potential allows for the detection and analysis of mitochondrial depolarization in cells.
Used in Detection of Mitochondrial Depolarization:
JC-1 is used as a detection tool for monitoring the collapse of ΔΨm induced by uncouplers of mitochondrial respiration. This is important for studying the effects of various substances on mitochondrial function.
Used in Multidrug-Resistance Cell Assays:
JC-1 is used as a functional assay tool for multidrug-resistance cells. It helps in understanding the mechanisms behind drug resistance and the role of mitochondria in this process.
Used in Apoptosis-Related Mitochondrial Modification Studies:
JC-1 is used as a research tool for studying apoptosis-related mitochondrial modifications. It aids in investigating the role of mitochondria in programmed cell death and the effects of various factors on this process.

Biochem/physiol Actions

Product does not compete with ATP.

InChI:InChI=1/C47H73N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-29-38-48-42-34-27-25-32-40(42)46(3,4)44(48)36-31-37-45-47(5,6)41-33-26-28-35-43(41)49(45)39-30-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h25-28,31-37H,7-24,29-30,38-39H2,1-6H3;(H,2,3,4,5)/q+1;/p-1

Upstream product

• 75-03-6 ethyl iodide 
• 3237-62-5 5,6-dichloro-1-ethyl-2-methyl benzimidazole 
• 80-40-0 ethyl ester of p-toluenesulfonic acid 
• 4751-25-1 2-(2'-anilinoethenyl)-5,6-dichloro-1,3-diethylbenzimidazole 

Relevant articles and documents

MACROPHAGE IDENTIFICATION AGENT, AND IDENTIFICATION METHOD, SORTING METHOD, EVALUATION METHOD, SCREENING METHOD AND KIT USING THE MACROPHAGE IDENTIFIER AGENT

An object of the present invention is to provide an identification method for a macrophage subtype using an organic compound. Another object of the present invention is to provide a macrophage identification agent containing an organic compound in which a spectral characteristic obtained when the organic compound is added is different depending on a macrophage subtype. Still another object of the present invention is to provide an evaluation method for a macrophage subtype using a macrophage identification agent, an analysis method for correlation between a macrophage subtype and a test substance, a screening method for correlation between a macrophage subtype and a test substance, and a kit. An identification method for a macrophage subtype utilizing that a spectral characteristic obtained with an organic compound added is different depending on a macrophage subtype is provided.

The structure of benzimidazole cyanine dyes, their spectroscopy and their performance in photographic emulsions

The multi-step synthesis of two benzimidazole trimethine cyanines as possible green sensitizing dyes (Gs3-1, Gs3-2) is reported. Compounds were characterized from UV-Vis, mass spectroscopic and NMR data. The charge density distributions of the two compounds, calculated using the semi-empirical MOPAC 93 package, are in agreement with assignments in NMR spectroscopy. Sensitizing properties of the cyanine dyes were evaluated in actual photographic T-grain emulsions. VCH Verlagsgescllschaft mbH, 1997.