Bio-solvents change regioselectivity in the synthesis of disaccharides using Biolacta β-galactosidase
Bio-solvents are good alternative solvents that avoid the use of classical organic solvents when performing enzymatic reactions. A noticeably change in regioselectivity was observed in the synthetic behaviour of Biolacta β-galactosidase using bio-solvents derived from dimethylamide and glycerol as co-solvents. Under these conditions, the enzyme changes its well known tendency to produce β-(1→4) to β-(1→6) disaccharides. An evaluation of the bio-solvent concentration and the effects of the non proteic additives in commercially available Biolacta β-galactosidase was undertaken in order to optimize the reaction conditions to improve the yield of the β-(1→6) product.
Pérez-Sánchez, María,Sandoval, Manuel,Hernáiz, María J.
experimental part
p. 2141 - 2145
(2012/03/27)
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