- Photoregulation of Gene Expression with Amantadine-Modified Caged siRNAs through Host–Guest Interactions
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RNA interference is an essential and powerful tool for targeting and verifying specific gene functions. Conditional control of small interfering RNA (siRNA) activity, especially using light activation, is a potential method for regulating target gene expr
- Zhang, Jinhao,Jing, Nannan,Fan, Xinli,Tang, Xinjing
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p. 14002 - 14010
(2020/10/02)
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- A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression
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Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3′-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, i
- Chen, Lei,Li, Jinbo,Sun, Yu.,Zhang, Yan
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p. 627 - 630
(2020/01/29)
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- Photochemical regulation of gene expression using caged siRNAs with single terminal Vitamin E modification
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Caged siRNAs with a single photolabile linker and/or vitamin E (vitE) modification at the 5′ terminal were rationally designed and synthesized. These virtually inactive caged siRNAs were successfully used to photoregulate both firefly luciferase and GFP g
- Ji, Yuzhuo,Yang, Jiali,Wu, Li,Yu, Lijia,Tang, Xinjing
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p. 2152 - 2156
(2016/02/18)
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- A rapid 1,2-dihydroxylation of alkenes using a lipase and hydrogen peroxide under microwave conditions
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The combined advantages of using an enzyme immobilized lipase from Pseudomonas sp [PSLG6], hydrogen peroxide, ethyl acetate and microwave irradiation for the dihydroxylation of olefins are reported.
- Sarma, Kuladip,Borthakur, Naleen,Goswami, Amrit
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p. 6776 - 6778
(2008/02/13)
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- Photocleavable circular oligonucleotides
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The photocleavable cyclic oligonucleotide according to the invention is the one that possesses a base sequence having the hybridization ability toward DNA or RNA to targeted, and is further provided with the structure cyclized by a photocleavable group. Accordingly, the photocleavable cyclic oligonucleotide according to the invention, after having been introduced in vivo, is hardly susceptible to the nuclease decomposition reaction owing to its cyclic structure and thus it is capable of diffusing toward the predetermined sites in vivo with sufficient time. Moreover, by being irradiated with the light at an appropriate wavelength after a predetermined period of time, the photocleavable group as described above is cleaved photochemically, thus cutting the predetermined bond. This permits the oligonucleotide that was cyclic to be a linear oligonucleotide which expresses the function of an antisense oligonucleotide.
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- Behavior modifying compounds
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Derivatives of carbonyl-group containing behavior modifying compounds, in which said carbonyl group has been converted to a photolabile group which regenerates the carbonyl group on exposure to radiation, are of value in various methods for the control of
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- Antibacterial Benzisoxazolones. An Unusual Rearrangement Product from o-Nitrostyrene Oxide en Route to the Photolabile Carbonyl Protecting Group (o-Nitrophenyl)ethylene Glycol
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In the process of synthesizing the known, photolabile protecting group (o-nitrophenyl)ethylene glycol, we uncovered an acid-mediated rearrangement of o-nitrostyrene oxide to 1-(hydroxymethyl)-2,1-benzisoxazol-3(1H)-one.The structure was ascertained by IR, UV, 1H NMR, 13C NMR, MS, and X-ray crystallography.Also the benzisoxazolone was prepared by independent synthesis from an o-nitrobenzoate.Another novel transformation uncovered was the conversion of o-nitrostyrene oxide to 2-(o-nitrophenyl)ethanol with use of potassium hydroxide and 18-crown-6.Similiar chemistry was insignificant with p-nitrostyrene oxide.The benzisoxazolone exhibited antibacterial and antileukemic activity (in vitro).
- Wierenga, Wendell,Harrison, Allen W.,Evans, Bruce R.,Chidester, Connie G.
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p. 438 - 442
(2007/10/02)
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- o-Nitrophenylethylene glycol as photoremovable protective group for aldehydes and ketones: syntheses, scope, and limitations
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The preparation of o-nitophenylethylene glycol is described along with its application as a photolabile protective group for aldehydes and ketones.Formation of the acetals and ketals is achieved in good to high yields in the usual manner and deprotection is carried out in fair to high yield, by photolysis at 350 nm in an inert solvent such as benzene.Because of the particular nature of the present protective group, its stability to basic and acidic conditions has been examined and is reported to complete the scope and limitations aspect.From a mechanistic point of view, the isolation and characterisation of o-nitroso-α-hydroxyacetophenone as the spent reagent demonstrates a mechanistic link with the known o-nitrobenzaeldehyde to o-nitrobenzoic acid photorearrangement.
- Gravel, D.,Herbert, J.,Thoraval, D.
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p. 400 - 410
(2007/10/02)
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- Substituted dioxolanes and photosensitive and degradable polymers useful in imaging
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Substituted dioxolanes are provided having the formula STR1 wherein R1 and R2 are H or a phenyl group substituted with up to 5 substituents of lower alkyl or nitro, with the proviso that at least one of F1 and R2/sup
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