- Hydrophilic carotenoids: Surface properties and aggregation behavior of the potassium salt of the highly unsaturated diacid norbixin
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The oft-claimed 'good' water solubility of the food color norbixin (3) could not be confirmed. In contrast, the potassium salt 5 of norbixin formed suitable dispersions. The surface and aggregation properties of salt 5 were investigated and compared with
- Breukers, Stefanie,pstad, Christer L.,Sliwka, Hans-Richard,Partali, Vassilia
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- Studies on interaction of norbixin with DNA: Multispectroscopic and in silico analysis
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The interaction of food colorant norbixin with calf thymus DNA (CTDNA) was investigated through UV-Visible spectroscopy, Fourier Transform Infrared (FTIR), Circular Dichroism (CD), Nuclear Magnetic Resonance (NMR), DNA melting studies, electrophoretic analysis, histological staining technique and molecular docking studies. The results indicated that norbixin interacted with CTDNA by partial intercalation mode. The binding constant (K) of norbixin with CTDNA was calculated to be 5.08 × 105 Mol-1 L. FTIR and CD studies were coupled with 1H NMR spectra revealed that norbixin intercalates partially and binds to the groove's, phosphate group, deoxyribose sugar of DNA and also induces conformational transition of B-form to A-form DNA. Agarose gel electrophoretic and histological staining technique results further prove that, norbixin specifically binds to the DNA in the cell. Moreover, molecular docking studies on the specific binding of norbixin with CTDNA have exhibited lowest conformation energy score of -3.2. Therefore, this food colorant has the ability to interact with DNA and it could emerge as a promising class of natural DNA targeted therapeutic.
- Anantharaman, Amrita,Priya, Rajendra Rao,Hemachandran, Hridya,Sivaramakrishna, Akella,Babu, Subramanian,Siva, Ramamoorthy
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- NOVEL INTERMEDIATES FOR PREPARING NORBIXIN OR BIXIN ETHYL ESTER AND METHODS THEREOF
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The present invention relates to a method for preparing a carotenoid compound, norbixin or bixin ethyl ester, from a novel intermediate compound. When using the novel intermediate compound, norbixin or bixin ethyl ester can be prepared in high yield through a simple process.
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Paragraph 0146; 0148-0152
(2020/10/13)
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- NOVEL INTERMEDIATE COMPOUND FOR MANUFACTURING BIXIN ETHYL ESTER AND METHOD OF MANUFACTURING THE SAME
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The present invention relates to a method of manufacturing norbixin or bixin ethyl ester, which is a carotenoid-based compound, from a novel intermediate compound. When the novel intermediate compound is used, it is possible to manufacture norbixin or bixin ethyl ester in high yield through a simple process.
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- Thermal degradation kinetics of bixin in an aqueous model system
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The kinetics of the thermal degradation of the natural cis carotenoid bixin in a water/ethanol (8:2) solution was studied as a function of temperature (70-125°C), using high-performance liquid chromatography. The curves for the decay of bixin and formation of products (e.g., di-cis and all-trans isomers and a C17 degradation compound) did not adjust well to a first-order rate law, but very good fits were obtained using a biexponential model. This mathematical modeling gave the rate constant values for the formation of the primary products from bixin, and the energy barrier for each step was obtained. The di-cis isomers were formed immediately (15 kcal/mol) together with the decay of bixin, followed by a slow consumption, indicating their role as reaction intermediates. In fact, the di-cis isomers could easily revert to bixin (Ea ≈ 3 kcal/mol) or yield the primary C17 degradation product, with an energy barrier of 6.5 kcal/mol. In turn, 24 kcal/mol was necessary for the Bix - all-trans step, explaining its slower formation.
- Rios, Alessandro De O.,Borsarelli, Claudio D.,Mercadante, Adriana Z.
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p. 2307 - 2311
(2007/10/03)
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