- Synthesis of Arylazide- and Diazirine-Containing CrAsH-EDT2 Photoaffinity Probes
-
Two photo-crosslinking biarsenical (CrAsH-EDT2)-modified probes were synthesized that are expected to be useful tools for tetracysteine-labeled proteins to facilitate the co-affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH-EDT2 and N1-(4-azido-2-nitrophenyl)hexane-1,6-diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3-related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.
- Syeda, Shameem S.,Rice, Daren,Hook, Derek J.,Heckert, Leslie L.,Georg, Gunda I.
-
p. 233 - 241
(2016/04/26)
-
- Azido-triazines
-
Novel compounds having the structural formula STR1 where B is NR, O, S, STR2 or --CH2 ; (M)n is a saturated or unsaturated aliphatic hydrocarbon chain; X is NR, O or S; A is SO2 R, CN, NO2 or H; Y is NR2, N3, halogen or SH; R is H and/or a lower alkyl group; D is N3 or halogen and n is 1-12.
- -
-
-