64309-09-7Relevant articles and documents
Synthesis of Arylazide- and Diazirine-Containing CrAsH-EDT2 Photoaffinity Probes
Syeda, Shameem S.,Rice, Daren,Hook, Derek J.,Heckert, Leslie L.,Georg, Gunda I.
, p. 233 - 241 (2016/04/26)
Two photo-crosslinking biarsenical (CrAsH-EDT2)-modified probes were synthesized that are expected to be useful tools for tetracysteine-labeled proteins to facilitate the co-affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH-EDT2 and N1-(4-azido-2-nitrophenyl)hexane-1,6-diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3-related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.