- Method for synthesizing 3 - (N - morpholinyl) -2 - hydroxyl propanesulfonic acid without solvent
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The invention relates to a method for synthesizing 3 - (N - morpholinyl) -2 - hydroxyl propanesulfonic acid without a solvent, and belongs to the technical field of organic synthesis. To the method, morpholine and 3 -chloro -2 -hydroxypropanesulfonate are adopted, the reaction is conducted under the condition of no solvent, and 3 - (N - morpholinyl) -2 - hydroxypropanesulfonic acid is obtained after the reaction is finished. Compared with the original 3 - (N - morpholinyl) -2 - hydroxypropanesulfonic acid production process, the raw materials are easily available, the operation is simple, the process yield is high and stable, and the product quality is excellent. The problems that the yield is low and the process is complex in the prior art are solved.
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Paragraph 0025-0055
(2021/11/10)
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- ENHANCING AGENT FOR DETECTION OF ANALYTE IN SPECIMEN, AND METHOD FOR DETECTING ANALYTE IN SPECIMEN
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Detection rapidly and with high detection intensity is accomplished while blackening is inhibited during silver enhancement to detect an analyte in a specimen. One embodiment of the present invention provides an enhancing agent to be used for silver enhancement in detection of an analyte in a specimen by metal labeling and silver enhancement, the enhancing agent including: (a) a silver-containing compound,(b) a silver ion-reducing agent, and(c) a reaction rate controller, wherein the reaction rate controller (c) is a compound selected from the group consisting of compounds represented by the following formula (I): (wherein: R1, R2 and R3 each independently represent a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms, with the proviso that R1, R2 and R3 are not all hydrogen atoms, or R1 and R2 form a 5-membered ring or 6-membered ring and R3 represents a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms;X represents a divalent hydrocarbon group of 1 to 3 carbon atoms; andn is an integer of 1 to 3).
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- 3-(N-morpholine)-2-hydroxyl propanesulfonic acid synthesizing technology
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The invention relates to a 3-(N-morpholine)-2-hydroxyl propanesulfonic acid synthesizing technology, and discloses a preparation method of a N-substituted morpholine organic compound. The preparation method comprises the following steps that cyclodehydration is conducted to obtain morpholine, neutralizing, filtering, rectifying and synthesizing are conducted to obtain 3-(N-morpholine)-2-hydroxyl propanesulfonic acid, and chromatography and purification are conducted to obtain the final product. According to the method, reaction conditions are mild, operation is easy and convenient, the safety is high, industrialization is easy to achieve, and the yield is stably 95% or above; in addition, reagents are low in cost and easy to obtain, and therefore obvious cost and technique advantages are achieved.
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Paragraph 0011; 0012; 0013; 0014
(2017/04/28)
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- Stabilizing labeled antibody using amino acids
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The present invention relates to a method for stabilizing a labeled antibody in a solution, in which the labeled antibody is stabilized by allowing the labeled antibody to be present together with at least one of amino acid and a derivative thereof in the solution.
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- Amino-hydroxy-alkyl sulfonic acid zwitterions
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Novel amino-hydroxy-alkylene sulfonic acid zwitterions are described which have the general formulas STR1 wherein A and B are each independently a hydrogen atom, an aliphatic, cycloaliphatic or hydroxyaliphatic group, N is one or two, and R=n is a cyclic radical comprising one or two ring nitrogen atoms, zero or one ring oxygen atoms and the remainder of the ring being carbon atoms, and water soluble salts of said acids. These organo-sulfonic acid compounds are useful as hydrogen ion buffers in biological research and particularly for tissue culture.
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