- Bifunctional small molecule compound capable of activating enzyme catalytic reaction, and applications thereof
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The present invention relates to a catalytic reaction, particularly to a bifunctional small molecule compound capable of activating an enzyme catalytic reaction, and applications thereof, wherein thebifunctional small molecule compound is C5-10 carboxylic acid, C5-10 fluorocarboxylic acid, aromatic heterocyclic ring substituted carboxylic acid, heterocyclic ring substituted carboxylic acid, a C5-10 carboxylic acid amide derivative, a C5-10 fluorocarboxylic acid amide derivative, an aromatic heterocyclic ring substituted carboxylic acid amide derivative or a heterocyclic ring substituted carboxylic acid amide derivative substituted with at least a catalytic group, wherein the carboxyl group or the amide derivative is used as an anchoring group. According to the present invention, with themethod, the number of the introduced catalytic groups cannot be limited, and the orientation of the catalytic functional group and the fine adjustment of the distance from the active center can be achieved, such that the H2O2 activation efficiency can be well enhanced, and the catalytic activity and the stereoselectivity can be improved.
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Paragraph 0066-0068; 0072; 0075-0081; 0082-0086
(2019/10/01)
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- Dual-Functional Small Molecules for Generating an Efficient Cytochrome P450BM3 Peroxygenase
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We report a unique strategy for the development of a H2O2-dependent cytochrome P450BM3 system, which catalyzes the monooxygenation of non-native substrates with the assistance of dual-functional small molecules (DFSMs), such as N-(ω-imidazolyl fatty acyl)-l-amino acids. The acyl amino acid group of DFSM is responsible for bounding to enzyme as an anchoring group, while the imidazolyl group plays the role of general acid–base catalyst in the activation of H2O2. This system affords the best peroxygenase activity for the epoxidation of styrene, sulfoxidation of thioanisole, and hydroxylation of ethylbenzene among those P450–H2O2 system previously reported. This work provides the first example of the activation of the normally H2O2-inert P450s through the introduction of an exogenous small molecule. This approach improves the potential use of P450s in organic synthesis as it avoids the expensive consumption of the reduced nicotinamide cofactor NAD(P)H and its dependent electron transport system. This introduces a promising approach for exploiting enzyme activity and function based on direct chemical intervention in the catalytic process.
- Ma, Nana,Chen, Zhifeng,Chen, Jie,Chen, Jingfei,Wang, Cong,Zhou, Haifeng,Yao, Lishan,Shoji, Osami,Watanabe, Yoshihito,Cong, Zhiqi
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p. 7628 - 7633
(2018/04/02)
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- Development of imidazole alkanoic acids as mGAT3 selective GABA uptake inhibitors
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A new series of potential GABA uptake inhibitors starting from of 1H-imidazol-4-ylacetic acid with the carboxylic acid side chain originating from different positions and varying in length have been synthesized and tested for the inhibitory potency at the
- Hack, Silke,W?rlein, Babette,H?fner, Georg,Pabel, J?rg,Wanner, Klaus T.
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experimental part
p. 1483 - 1498
(2011/05/11)
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