- The synthesis of oligoribonucleotides VI. The synthesis of a hexadecamer by a block condensation approach
-
The synthesis of the 5'-monomethoxytrityl-2'-tert-butyldimethylsilyl-3'-levulinylribonucleosides 4 of the four major bases involved in the phosphite procedure for the synthesis of oligoribonucleotides is described along with the 2',3'-isomeric derivatives
- Ogilvie, Kelvin K.,Nemer, Mona J.
-
p. 1389 - 1397
(2007/10/02)
-
- The chemical synthesis of oligoribonucleotides. IX. A comparison of protecting groups in the dichloridite procedure
-
A series of phosphorodichloridites has been prepared incorporating the most commonly used phosphate protecting groups in oligonucleotide synthesis.The groups incude trichloroethyl, tribromoethyl, cyanoethyl, benzyl, methyl, p-chlorophenyl, and nitrophenetyl.The trichloroethyl and the nitrophenethyl appear to be the most stable groups while the cyanoethyl and methyl offer specific advantages.The benzyl and p-chlorophenyl groups are subject to limitations on their utility.Condensations can be carried out in a range of solvents incuding THF, pyridine, and DMF and at temperatures from -78 deg C to 20 deg C with a slight drop in yield with increasing temperature, at least for dinucleotide condensations.
- Ogilvie, Kelvin K.,Theriault, Nicole Y.,Seifert, Jan-Marcus,Pon, Richard T.,Nemer, Mona J.
-
p. 2686 - 2693
(2007/10/02)
-