A novel method for the preparation of nucleoside triphosphates from activated nucleoside phosphoramidates
A novel method for the preparation of nucleoside triphosphates has been developed. The strategy employs a highly reactive pyrrolidinium phosphoramidate zwitterion intermediate that undergoes efficient coupling with tris(tetra-n-butylammonium) hydrogen pyrophosphate to generate nucleoside triphosphate.
Wu, Weidong,Meyers, Caren L. Freel,Borch, Richard F.