- A superparamagnetic iron oxide nano MRI probe and its preparation method and application thereof
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The invention relates to a superparamagnetic iron oxide nano MRI (magnetic resonance imaging) probe and a preparation method and application thereof. A chemical formula of the superparamagnetic iron oxide nano MRI probe is shown in the specification, wherein SPIO refers to superparamagnetic iron oxide, CTX refers to scorpion chloride toxins, and n refers to degree of polymerization and is an arbitrary integer from 4 to 200. The novel superparamagnetic iron oxide nano MRI probe is prepared successfully, a magnetic resonance imaging law of a nanoparticle solution of the MRI probe is identical to that of an SPIO solution, and the magnetic resonance imaging law refers to that along with increase of concentration of iron in the solution, signal intensity of a T1WI sequence is increased, and signal intensity of a T2WI sequence is reduced. The superparamagnetic iron oxide nano MRI probe has no evident cytotoxicity to human hepatocellular carcinoma cell line HepG2 and can be used for early detection of hepatocellular carcinoma.
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Paragraph 0022; 0048-0049
(2018/11/22)
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- Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations
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Gold nanoparticles decorated with full-length sialic acid terminated complex bi-antennary N-glycans, synthesized with glycans isolated from egg yolk, were used as a sensor for the detection of both recombinant hemagglutinin (HA) and whole influenza A virus particles of the H1N1 subtype. Nanoparticle aggregation was induced by interaction between the sialic acid termini of the glycans attached to gold and the multivalent sialic acid binding sites of HA. Both dynamic light scattering (DLS) and UV/Vis spectroscopy demonstrated the efficiency of the sensor, which could detect viral HA at nanomolar concentrations and revealed a linear relationship between the extent of nanoparticle aggregation and the concentration of HA. UV/Vis studies also showed that these nanoparticles can selectively detect an influenza A virus strain that preferentially binds sialic acid terminated glycans with α(2→6) linkages over a strain that prefers glycans with terminal α(2→3)-linked sialic acids.
- Poonthiyil, Vivek,Nagesh, Prashanth T.,Husain, Matloob,Golovko, Vladimir B.,Fairbanks, Antony J.
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p. 708 - 716
(2016/07/06)
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- Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations
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Gold nanoparticles decorated with full-length sialic acid terminated complex bi-antennary N-glycans, synthesized with glycans isolated from egg yolk, were used as a sensor for the detection of both recombinant hemagglutinin (HA) and whole influenza A virus particles of the H1N1 subtype. Nanoparticle aggregation was induced by interaction between the sialic acid termini of the glycans attached to gold and the multivalent sialic acid binding sites of HA. Both dynamic light scattering (DLS) and UV/Vis spectroscopy demonstrated the efficiency of the sensor, which could detect viral HA at nanomolar concentrations and revealed a linear relationship between the extent of nanoparticle aggregation and the concentration of HA. UV/Vis studies also showed that these nanoparticles can selectively detect an influenza A virus strain that preferentially binds sialic acid terminated glycans with α(2→6) linkages over a strain that prefers glycans with terminal α(2→3)-linked sialic acids.
- Poonthiyil, Vivek,Nagesh, Prashanth T.,Husain, Matloob,Golovko, Vladimir B.,Fairbanks, Antony J.
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p. 708 - 716
(2016/09/09)
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- Comparison of the labelling characteristics of mercaptoacetyltriglycine (MAG3) with different S-protective groups
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A number of different thiol protective groups have been synthesized and attached to mercaptoacetyltriglycine (MAG3) ligand. The newly made MAG3 analogues were labelled with 99mTc by direct labelling under alkaline condition and by stannous tartrate exchange labelling method. In the latter method, the amount of the ligand, reaction temperature and pH varied and their effects on the labelling efficiencies were studied. Radiochemical purities of 51% to 70%, 58% to 75% and 46% to 81% respectively, were obtained by radio-HPLC analysis for the studied MAG3 precursors when, 0.1 mg, 0.4 mg and 1.6 mg of the ligand was used and labelling was performed at both low temperature (70°C) and pH (pH 3). All the studied ligands were efficiently labelled with 99mTc (up to 99%) when heated for 10 min at pH 9 and 100°C. The labelling efficiency obtained by the direct labelling method for MAG3 analogues varied from 32% to 94% and was in all cases lower than after the exchange labelling at pH 9 and at 100°C. It was observed that the radiochemical purities can be improved significantly by heating the 'direct labelling mixture' at elevated temperature.
- Okarvi, Subhani M.,Adriaens, Paul,Verbruggen
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p. 853 - 874
(2007/10/03)
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