- Attempts for the Application of Thermitase as a Catalyst for Peptide Bond Formation
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The utility of thermitase, a thermophilic proteolytic enzyme from Thermoactinomyces vulgaris, as a catalyst for peptide synthesis has been studied using several Nα-benzyloxycarbonyl dipeptide esters as carboxyl components and Leu-NH2 as nucleophile.Couplings were most successfull with substrates containing Met, Leu, Ala, Phe, Tyr in P1-position, and Val, Ala, Pro in P2, whereas the substrates with Val, Ile, Pro in P1 failed to couple. - Keywords: Enzymatic peptide synthesis; Thermitase
- Koennecke, Andreas,Jakubke, Hans-Dieter
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