CONVERSION OF 17O/18O LABELLED δ-(L-α-AMINOADIPYL)-L-CYSTEINYL-D-VALINE TO 17O/18O LABELLED ISOPENICILLIN IN A CELL-FREE EXTRACT OF CEPHALOSPORIUM ACREMONIUM. A STUDY BY 17O-NMR SPECTROSCOPY AND MASS SPECTROMETRY.
δ-(L-α-Amino17O/18O>-adipyl)-L-cystinyl-D-valine was converted into isopenicillin N in cell-free extracts of Cephalosporium acremonium with no loss of 17O/18O label as shown by 17O NMR spectroscopy and mass spectrometry.Incubation of unlabelled tripeptide in a cell-free system containing 17O/18O enriched water produced isopenicillin N with no incorporation of 17O/18O.
Adlington, Robert M.,Aplin, Robin T.,Baldwin, Jack E.,Chakravarti, Bulbul,Field, Leslie D.,et. al.
Get Best Price for85989-38-4(2S,5R,6R)-6-((S)-5-Ethoxycarbonylamino-5-methoxycarbonyl-pentanoylamino)-3,3-dimethyl-7-oxo-4-thia-1-aza-bicyclo[3.2.0]heptane-2-carboxylic acid methyl ester