Synthesis of oligodeoxyribonucleotides containing hydroxymethylphosphonate bonds in the phosphoramidite method and their hybridization properties
A set of diastereomeric TpT phosphoramidite building blocks, 1-f and 1-s, containing an alkaline-labile hydroxymethylphosphonate (HMP) linkage were synthesized. Dodecadeoxynucleotides incorporating the HMP bond were also synthesized by use of each stereoc