- Optimization of the enzymatic synthesis of O-glycan core 2 structure by use of a genetic algorithm
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The enzymatic synthesis of Gal-β1,3[GlcNAc-β1,6]-GalNAc-α1-OBn (core 2-Bn) using a multi-enzyme system consisting of a β-galactosidase (EC 3.2.1.23) from bovine testes and a recombinant core 2 β1,6-GlcNAc transferase (C2GnT, EC 2.4.1.102) was empirically optimized by the use of a genetic algorithm. After variation of seven relevant parameters and performance of 56 experiments, two local maxima regarding the selection criterias could be found after four generations of optimization. The selectivity of core 2-Bn formation showed values up to 90%.
- Hoh, Christoph,Dudziak, Gregor,Liese, Andreas
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p. 1031 - 1034
(2007/10/03)
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- In situ generated O-glycan core 1 structure as substrate for Gal(β1- 3)GalNAc β-1,6-GlcNAc transferase
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β-Galactosidase from bovine testes was used in a one pot reaction together with a recombinant β-1,6-GlcNAc transferase for the synthesis of GlcNAc(β1-6)GalNAc(α1-OBn) (core 6-Bn). The galactosidase, which reversibly links galactose via a (β1-3)
- Dudziak, Gregor,Zeng, Steffen,Berger, Eric G.,Gallego, Ricardo Gutierrez,Kamerling, Johannis P.,Kragl, Udo,Wandrey, Christian
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p. 2595 - 2598
(2007/10/03)
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- Synthetic mucin fragments: benzyl 2-acetamido-6-O-(2-acetamido-2-deoxy-beta- D-glucopyranosyl)-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D- galactopyranoside and benzyl 2-acetamido-6-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)- 3-O-[6-O-(2-acetamido-2-d
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Glycosylation of benzyl 2-acetamido-4,6-O-benzylidene-2-deoxy-alpha-D- galactopyranoside with 2,3,4,6-tetra-O-acetyl-alpha-D-galactopyranosyl bromide, catalyzed by mercuric cyanide, afforded benzyl-2-acetamido-4,6-O-benzylidene-2-deoxy-3- O-(2,3,4,6-tetra
- Piskorz,Abbas,Matta
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p. 115 - 124
(2007/10/02)
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