10019-03-1 Usage
Uses
Used in Biochemical Assays:
Naphthol AS-BS phosphate is used as a substrate for detecting and quantifying alkaline phosphatase activity, enabling the study of enzyme kinetics and the diagnosis of diseases related to abnormal enzyme levels.
Used in Enzyme-Linked Immunosorbent Assays (ELISAs):
In ELISAs, Naphthol AS-BS phosphate serves as a colorimetric indicator, allowing for the detection and quantification of specific antigens or antibodies, which is crucial for biomedical research and diagnostic applications.
Used in Biomedical Research:
Naphthol AS-BS phosphate is utilized in various research applications to study the activity and function of alkaline phosphatase, contributing to a better understanding of enzyme mechanisms and their role in disease processes.
Used in Diagnostic Applications:
In the medical field, Naphthol AS-BS phosphate is employed for diagnosing diseases associated with changes in alkaline phosphatase levels, such as liver or bone disorders, by providing a quantitative measure of enzyme activity.
Safety Precautions:
Naphthol AS-BS phosphate should be handled and disposed of with care, as it can cause irritation to the eyes, skin, and respiratory tract, and may be harmful if ingested or inhaled. Proper safety measures, including the use of personal protective equipment and adherence to disposal protocols, are essential to minimize potential health risks.
Check Digit Verification of cas no
The CAS Registry Mumber 10019-03-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,0,1 and 9 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 10019-03:
(7*1)+(6*0)+(5*0)+(4*1)+(3*9)+(2*0)+(1*3)=41
41 % 10 = 1
So 10019-03-1 is a valid CAS Registry Number.
InChI:InChI=1/C17H13N2O7P/c20-17(18-13-6-3-7-14(10-13)19(21)22)15-8-11-4-1-2-5-12(11)9-16(15)26-27(23,24)25/h1-10H,(H,18,20)(H2,23,24,25)
10019-03-1Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
