125454-31-1Relevant articles and documents
Large-scale Enrichment of Sulfur-containing Acid in Acetonitrile Using Triazine Analog as the Derivatizing Reagent for Desorption from Humic-fraction-modified Silica Gel
Hu, Shun-Wei,Chen, Shushi
, p. 703 - 708 (2016)
The analog of triazine is used as the derivatizing reagent for enriching large-scale acid (e.g. amino acid) containing a sulfur atom on the humic-fraction-modified silica gel in acetonitrile and desorbing from the adsorbent in hexane, respectively. The percent yield of the chemical derivatization under alkaline conditions, ranging from about 8 to almost 100 %, is pH dependent, and varies significantly among these examined analytes, believed to be due to the structure of the analyte, not the derivatizing reagent. The percentage of enrichment, not reproducible under an aqueous environment and independent of the type of triazine analogs, reaches almost 100 % in all cases. The force leading to the adsorption is the complexation between carboxyl groups on analyte and a humic-fraction-modified adsorbent based on the adsorption equilibrium results. Consequently, these results are not reproducible under ethyl ether or methanol environments due to the competition for binding sites from solvent molecules.
A highly sensitive immunoassay for atrazine based on covalently linking the small molecule hapten to a urea–glutaraldehyde network on a polystyrene surface
Sai, Na,Sun, Wenjing,Wu, Yuntang,Sun, Zhong,Yu, Guanggui,Huang, Guowei
, p. 480 - 486 (2016)
A new enzyme-linked immunosorbent assay (ELISA) for atrazine was developed based on covalent bonding of the small molecule hapten, 2-mercaptopropionic acid-4-ethylamino-6-isopropylamino-1,3,5-triazine (MPA–atrazine), to urea-glutaraldehyde (UGA)-treated microtiter plates. In this assay, the microtiter plate surface was treated with the UGA network to both introduce amino groups, which were used to cross-link with the hapten carboxylate groups, and efficiently prevent non-specific adsorption of antibodies, which successfully eliminated the time-consuming routine blocking step. Compared with HNO3-H2SO4-APTES-hapten coated ELISA (modified with a HNO3-H2SO4-APTES mixture and covalent-linked hapten) and conventional ELISA (coated with hapten–carrier protein conjugates), the novel ELISA format increased the sensitivity by approximately 3.5-fold and 7.5-fold, respectively, and saved 2.5?h and 34?h of coating hapten time, respectively. The method's 50% inhibition concentration for atrazine was 5.54?ng?mL??1, and the limit of detection was 0.16?ng?mL??1 after optimization of reaction conditions. Furthermore, the ELISA was adapted for analysis of atrazine in corn, rice, and water samples, demonstrating recoveries of 90%–108%. Thus, the assay provides a convenient alternative to conventional, laborious immunoassays for routine supervision of residue detection in food and the environment.
Immunochromatographic dipstick assay format using gold nanoparticles labeled protein-hapten conjugate for the detection of atrazine
Kaur, Jasdeep,Singh, K. Vikas,Boro, Robin,Thampi,Raje, Manoj,Varshney, Grish C.,Suri, C. Raman
, p. 5028 - 5036 (2007)
The present study describes a lateral-flow-based dipstick immunoassay format using a novel hapten-protein-gold conjugate for the rapid screening of atrazine in water samples. The immunoassay is based on the competitive inhibition, in which a newly develop
Agent orange herbicides, organophosphate and triazinic pesticides analysis in olive oil and industrial oil mill waste effluents using new organic phase immunosensors
Martini, Elisabetta,Merola, Giovanni,Tomassetti, Mauro,Campanella, Luigi
, p. 358 - 365 (2015/01/08)
New immunosensors working in organic solvent mixtures (OPIEs) for the analysis of traces of different pesticides (triazinic, organophosphates and chlorurates) present in hydrophobic matrices such as olive oil were developed and tested. A Clark electrode w
