16052-06-5

Basic information

• Product Name: EPPS
• Synonyms: HEPPS(EPPS);4-(2-Hydroxyethyl)pi;HEPE;HEPPS 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic Acid;4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid HEPPS/EPPS/HEPE;4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid[HEPPS];4-Hydroxyerhylpiperazine-1-propanesulfonic acid
• CAS NO: 16052-06-5
• Molecular Formula: C₉H₂₀N₂O₄S
• Molecular Weight: 252.33
• EINECS: 240-198-8
• Product Categories: buffer;Pharmaceutical Intermediates;Miscellaneous Biochemicals;Biochemistry;Good's Buffers
• Mol File: 16052-06-5.mol
• Article Data: 1

Chemical Properties

• Melting Point: 237-239 °C(lit.)
• Appearance: White/Powder/Solid
• Density: 1.2684 (rough estimate)
• Refractive Index: 1.6800 (estimate)
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear, colorless
• PKA: 8.0(at 25℃)
• Water Solubility: Soluble in water. 25.2 g/L (20°C)
• BRN: 3957385
• CAS DataBase Reference: EPPS(CAS DataBase Reference)
• NIST Chemistry Reference: EPPS(16052-06-5)
• EPA Substance Registry System: EPPS(16052-06-5)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38-22
• Safety Statements: 24/25-36-26
• WGK Germany: 3
• RTECS: TM2649130
• TSCA: Yes
• HazardClass: IRRITANT
• Hazardous Substances Data: 16052-06-5(Hazardous Substances Data)

Usage

Chemical Properties

white crystalline powder

Uses

EPPS is a commonly used Good?s buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry.

Flammability and Explosibility

Notclassified

InChI:InChI=1/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

Synthetic route

copper hydroxide (20427-59-2) + 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) → N-(2-hydroxyethyl)-piperazine-N'-3-propane sulfonic acid copper(II) salt

Conditions	Yield
metal hydroxide dissoln. in org. acid soln. (pH 7 adjusting with additional org. acid); repeated dialysis against Cu(II)-salt soln., then against pure water;

cadmium(II) hydroxide + 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) → N-(2-hydroxyethyl)-piperazine-N'-3-propane sulfonic acid cadmium(II) salt

Conditions	Yield
metal hydroxide dissoln. in org. acid soln. (pH 7 adjusting with additional org. acid); repeated dialysis against Cd-salt soln., then against pure water;

4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) + tetra-n-butylphosphonium hydroxide (14518-69-5) → [TBP][EPPS]

Conditions	Yield
In water at 20℃;

tetramethyl ammoniumhydroxide (75-59-2) + 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) → C9H19N2O4S(1-)*C4H12N(1+)

Conditions	Yield
In water at 20℃; for 12h;

tetraethylammonium hydroxide (77-98-5) + 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) → C9H19N2O4S(1-)*C8H20N(1+)

Conditions	Yield
In water at 20℃; for 12h;

tetra(n-butyl)ammonium hydroxide (2052-49-5) + 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid (16052-06-5) → C9H19N2O4S(1-)*C16H36N(1+)

Conditions	Yield
In water at 20℃; for 12h;

Relevant articles and documents

METHODS AND COMPOSITIONS FOR RAPIDLY DECREASING EPIGENETIC AGE AND RESTORATION OF MORE YOUTHFUL FUNCTION

Disclosed are methods and compositions of reducing the epigenetic age of mammalian organism, especially an adult human of geriatric age. The methods provide for the proliferation of endogenous stem cells using mitochondrial fusion and a UCP2 blocker or other stimulants; supplying stem cells with nutrition to prevent cell cycle arrest; and removal of senescent somatic cells using senolytic treatments. The proliferation of endogenous neural stem cells after plaque removal for the treatment of Alzheimer's is also disclosed.