74561-18-5Relevant articles and documents
Determination of panthenol enantiomers in cosmetic preparations using an achiral-chiral–coupled column HPLC system
Hroboňová, Katarína,Lomenova, Anna
, p. 191 - 199 (2019/12/15)
A new high-performance liquid chromatography (HPLC) method for separation and determination of panthenol enantiomers in hair care products was developed. Two types of detectors, low-wavelength ultraviolet (UV) and polarimetric, were used. Optimized conditions consisted of coupled achiral, amino type, and chiral, amylose tris(3,5-dimethylphenylcarbamate), stationary phases, mixture of n-hexane/ethanol (60:40, v/v) as mobile phase under isocratic conditions and flow rate 0.8 cm3 min?1. The effect of column temperature on retention and resolution of enantiomers was studied. The analysis runtime was 10 minutes, and the average retention times for d- and l-panthenol were 7.10 ±0.1 minutes and 8.21 ±0.2 minutes, respectively. The resolution of enantiomers on coupled achiral-chiral columns was Rs = 2.7. The solid-phase extraction method was employed for extraction and purification of analytes. The validated method was selective, accurate, and linear (R2 >.998) over the concentration range of 0.001 to 1.0 mg cm?3 for both enantiomeric forms. The limits of detection (LOD) and quantitation (LOQ) of each enantiomer were 0.3 and 1.0 μg cm?3, respectively. The results demonstrated the occurrence of d-panthenol in hair care products.
