1510
Kunes, Jachym, Jirasko, Odlerova, Waisser:
temperature for 1 day, whereafter it was poured into a saturated sodium carbonate solution (100 ml).
The separated product was filtered off, washed consecutively with a saturated sodium carbonate solution
and water, and recrystallized from ethanol. The results are given in Table I.
4′-Isopropylthiobenzanilides 12–17 and 4′-Butylthiobenzanilides 18–22
To a refluxing mixture of a 4′-alkylbenzanilide (14 mmol) and pyridine (10 ml), phosphorus penta-
sulfide (7.1 mmol) was added portionwise and the reaction mixture was refluxed for 3–8 h. After
cooling, it was poured into water (100 ml). On the following day, the raw product was filtered off
and recrystallized from ethanol. The results are given in Table II.
Microbiological Evaluation
The microbiological tests were carried out on a semisynthetic liquid medium with proteins by Sula
(SEVAC, Prague). The following mycobacterial strains were used: Mycobacterium tuberculosis
H37Rv, Mycobacterium kansasii PKG 8, Mycobacterium avium No. 80/72 and Mycobacterium fortuitum
No. 1 023. The substances were added after dissolution in dimethyl sulfoxide. The resulting concen-
trations of the compounds in the substrate were: 7, 15, 30, 60, 125, 250, 500 and 1 000 µmol/l. The
minimum inhibition concentrations (MIC) were read after 15 days of incubation at 37 °C. The results
are summarized in Table IV.
Calculations
All calculations were carried out using the Multireg program for Microsoft Excel. Table V presents
a survey of the calculated contributions of the varied structural fragments to the activity.
The authors wish to thank Mrs D. Karlickova from the Department of Pharmaceutical Chemistry and
Drug Control, Faculty of Pharmacy, Charles University, for CHN elemental analysis and Mrs J. Zizkova
from the Department of Inorganic and Organic Chemistry for elemental analysis of sulfur and IR
spectra measurements. The work was supported by grant No. 35/95 of the Grant Agency of the Charles
University and by grant No. 203/96/0262 of the Grant Agency of the Czech Republic.
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Collect. Czech. Chem. Commun. (Vol. 62) (1997)