1
56
M.A. Olszewska, J.M. Roj / Phytochemistry Letters 4 (2011) 151–157
0
0
3
.8. 3,5,7,4 -Tetrahydroxy-3 -methoxyflavone-3-O-
b
-
D
-
derivative of
(R 18.10 min), derivative of
tive of -glucose (R 19.74 min). As the result, D
D
-galactose (R
t
16.96 min), derivative of
-glucose (R 18.48 min), and deriva-
-glucose was
-galactose in
L-galactose
glucopyranoside (5)
t
L
t
D
t
Pale yellow needles. TLC: R
f
0.41 (S-1). HPLC: R
): 255 (4.40), 272 (4.30), 322 (4.08), 367 (4.31);
: 268, 303, 362, 403; +AlCl +HCl:
70, 300, 358, 403; +NaOAc: 280, 328, 400; +NaOAc+H BO : 255,
70, 303, 360. For H and C NMR data see Tables 2 and 3.
t
21.39 min. UV
identified in the hydrolysates of 1–3, 5, 7, and 8, and
the hydrolysates of 4 and 6.
D
MeOH
max
l
+
2
2
nm (log e
NaOMe: 272, 328, 418; +AlCl
3
3
3
3
Acknowledgement
1
13
This work was financially supported by the Polish Ministry of
Science and Higher Education (grant project: N N405 398037).
0 0 00 000
.9. 3,5,7,4 -Tetrahydroxy-3 -methoxyflavone-3-O-[6 -O-(3 -
3
0
00
hydroxy-3 -methylglutaroyl)]-
6)
b-D-galactopyranoside, torminaloside
(
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Yellow amorphous solid. TLC: R
f
0.39 (S-1). HPLC: R
): 255 (4.41), 265 (4.33), 300 (4.11), 356 (4.32);
NaOMe: 271, 328, 415; +AlCl : 269, 279, 301, 360, 402;
AlCl +HCl: 267, 275, 300, 359, 400. +NaOAc: 275, 320, 382;
NaOAc+H
t
22.94 min.
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3
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: 256, 269, 300, 359. IR
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13
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30
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3
.10. Hydrolytic methods
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2
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3
.11. Determination of absolute configuration of monosaccharide
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2
0% (v/v) aqueous MeOH. The eluates containing sugars were
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3
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tate derivatives of the monosaccharides were analysed by HPLC,
and identified by comparison with the retention times of
derivatives of the standard samples obtained in the same manner: