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Pleas De ad l to o nn oT tr aa nd sj au cs t ti omn as rgins
Journal Name ARTICLE
Mycoplasma sp. contamination using the EZ-PCR mycoplasma
detection kit according to the manufacturer’s instructions.
Votruba, D. Řeha and P. Hobza, Chem. EurD. OJ,I2: 1000.140,3190/,C290D5T80-4174E
2
066.
8
. For recent examples of other organometallic derivatives of
DNA/RNA nucleobases, see: (a) P. R. Florindo, D. M. Pereira, P.
M. Borralho, M. F. M. Piedade, M. C. Oliveira, A. M. Dias, C. M. P.
Rodrigues and A. C. Fernandes, New. J. Chem., 2019, 43, 1195-
Crystal violet assay: Cells were sub-cultured into 96-well plates at a
density of 6250 cells per well in 100 µL of complete DMEM and left
overnight to allow the cells to attach. The next day culture media was
removed and replaced with fresh media containing test compounds
1
201; (b) M. I. P. S. Leitão, F. Herrera and A. Petronilho, ACS
(0–80 µM) dissolved in DMEM with a final concentration of 0.5% v/v
Omega, 2018, 3, 15653–15656; (c) A. Collado, M. Gómez-Gallego
and M. A. Sierra, Eur. J. Org. Chem. 2018, 1617–1623; (d) R
Kaczmarek, D Korczynski, K Krolewska-Golinska, K. A. Wheeler, F.
A. Chavez, A Mikus and R Dembinski, ChemistryOpen, 2018, 7,
237-247.
DMSO prepared from 50 mM stock solutions, except cisplatin which
was prepared as a 2 mM stock solution in phosphate buffered saline
(PBS). All cultures were incubated for 72 hrs prior to commencement
of the crystal violet assay as described below. The old media was
removed, cells washed with 100 μL of PBS, then 100 μL 4% v/v
paraformaldehyde was added. After 15 min this was removed and
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1
. H. V. Nguyen, A. Sallustrau, J. Balzarini, M. R. Bedford, J. C. Eden,
N. Georgousi, N. J. Hodges, J. Kedge, Y. Mehellou, C. Tselepis and
J. H. R. Tucker, J. Med. Chem., 2014, 57, 5817-5822.
0. J. L. Kedge, H. V. Nguyen, Z. Khan, L. Male, M. K. Ismail, H. V.
Roberts, N. J. Hodges, S. L. Horswell, Y. Mehellou and J. H. R.
Tucker, Eur. J. Inorg. Chem., 2017, 466-476.
1
00 μL of crystal violet solution (0.5% w/v in 10% v/v ethanol) added
and plates incubated for 20 min. Next the crystal violet solution was
removed, and plates washed with PBS (4 x 100 μL) before being
allowed to air dry for 20 min at room temperature. Finally, samples
were solubilized using 10 % v/v acetic acid before before measuring
the absorbance at 590 nm using a well-plate reader.
1
1
1. S. Gubin, S. Smirnova, L. Denisovich and A. Lubovich, J.
Organomet. Chem, 1971, 30, 243-255.
2. (a) P. Pigeon, S. Top, A. Vessieres, M. Huche, E. A. Hillard, E.
Salomon and G. Jaouen, J. Med. Chem, 2005, 48, 2814-2821; (b)
H. Z. S. Lee, O. Buriez, E. Labbe, S. Top, P. Pigeon, G. Jaouen, C.
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3. For a recent example, see: S. Khanapure, M. Jagadale, P.
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Acknowledgements
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J.H.R.T. acknowledges support from the Engineering and Physical
Sciences Research Council (EPSRC) for the award of a Leadership
Fellowship (grant number EP/G007578/1), the Leverhulme Trust for
a research grant entitled “Expanding the range and versatility of
ferrocene nucleic acids” (RPG-2013-100). The Centre for Chemical
and Materials Analysis at the University of Birmingham is
acknowledged for technical support. M.K.I acknowledges the
Ministry of Higher Education & Scientific Research in Iraq and The
University of Kirkuk for funding and support. We thank the EPSRC UK
National Crystallography Service at the University of Southampton
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173, 142-147.
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4. T. Hayashi, A. Ohno, S.-J. Lu, Y. Matsumoto, E. Fukuyo and K.
Yanagi, J. Am. Chem. Soc., 1994, 116, 4221-4226.
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Chem., 1984, 23, 1943-1945.
6. Y. S. Sohn, Schluete.Aw, Hendrick.Dn and H. B. Gray, Inorg.
Chem. 1974, 13, 301-304.
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(b) T. Kuwana, D. E. Bublitz and G. Hoh, J. Am. Chem. Soc., 1960,
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for the collection of the crystallographic data for 15.
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2, 5811-5817.
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8. J. C. Swarts, A. Nafady, J. H. Roudebush, S. Trupia and W. E.
Geiger, Inorg. Chemistry, 2009, 48, 2156-2165.
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