POLYMETHYLENE DERIVATIVES OF NUCLEIC BASES WITH ω-FUNCTIONAL GROUPS
73
for 30 min at 17000 g, dissolved in phosphate buffer 11. Kritzyn, A.M., Vesalainen, J., and Komissarov, V.V.,
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(
10 mM KH PO , pH 7.3), and dialyzed against the
2 4
same buffer.
1
1
2. Esteban-Gamboa, A., Balzarini, J., Esnouf, R.,
De Clercq, E., Camarasa, M.-J., and Perez-Perez, M.-J.,
J. Med. Chem., 2000, vol. 43, pp. 971–983.
The enzyme yield from 10 g of crude cells was
00−400 mg with specific activity of 45 U/mg (UP) and
20 U/mg (TP). Based on electrophoresis data, the
purity of the isolated proteins exceeded 95%. The
obtained values of specific activities agreed with the
published data [26].
3
1
3. Konstantinova, I.D., Leont’eva, N.A., Galegov, G.A.,
Ryzhova, O.I., Chuvikovsky, D.V., Antonov, K.V., Esi-
pov, R.S., Taran, S.A., Verevkina, K.N., Feofanov, S.A.,
and Miroshnikov, A.I., Bioorg. Khim., 2004, vol. 30,
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ACKNOWLEDGMENTS
1
1
1
4. Moghaddam, A., Zhang, H.T., Fan, T.P.D., Hu, D.E.,
Lees, V., Turley, H., Fox, S.B., Gatter, K.C., Harris, A.L.,
and Bicknell, R., Proc. Natl. Acad. Sci. USA, 1995,
vol. 92, pp. 998–1002.
We are grateful to A.R. Khomutov and S.N. Mi-
khailov for fruitful discussions of the results.
This work was supported by the Russian Foundation
for Basic Research, project nos. 06-04-48716-a and 04-
5. Matsushita, S., Nitanda, T., Furukawa, T., Sumizawa, T.,
Tani, A., Nishimoto, K., Akiba, S., Miyadera, K., Fuku-
shima, M., Yamada, Y., Yoshiba, H., Kanzaki, T., and
Akiyama, S., Cancer Res., 1999, vol. 59, pp. 1911–1916.
0
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RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY Vol. 34 No. 1 2008