Mechanism-based inactivation of serine proteases by dichlorocyclopropane fused lactone derivatives

Article abstract of DOI:10.1016/S0960-894X(96)00474-X

A dichlorocyclopropane fused lactone derivative was prepared as a novel mechanism-based inactivator of serine protease, α-chymotrypsin. The lactone derivative showed transient irreversible inhibitory activity toward α-chymotrypsin with the value of k(obsd)/[I] = 54 M^-1s^-1 and the enzyme activity recovered perfectly after 6 hours.