Paper
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6 J. Matic, F. Supljika, T. Tandaric, M. Duksi, P. Piotrowski,
ct-DNA was additionally sonicated and filtered through a 0.45 mm
filter.37 Polynucleotide concentration was determined spectro-
scopically by measuring the absorbance at the respective UV/Vis
maximum of the polynucleotide defined by the manufacturer, and
using the corresponding molar extinction coefficients, whereby
the concentration per nucleobase was obtained (traditionally
referred to as the concentration of phosphates).
For the study of interactions with DNA and RNA, aqueous
solutions of compounds were buffered to pH = 7.0 (Na cacodylate
buffer, I = 0.05 M). Spectrophotometric titrations were performed
by adding portions of polynucleotide solution into the solution of
the studied compound and CD experiments were performed by
adding portions of the compound stock solution into the solution
of the polynucleotide. In all titration experiments, dilution effects
were taken into account.
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2011, 9, 198–209.
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8 K. Klemm, M. Radic Stojkovic, G. Horvat, V. Tomisic,
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Thermal melting curves for ds-DNA, ds-RNA and their com-
plexes with studied compounds were determined as previously
described,30 by following the absorption change at 260 nm as a
function of temperature. The absorbance of the ligands was
subtracted from every curve and the absorbance scale was
normalized. Tm values are the midpoints of the transition
curves determined from the maximum of the first derivative
and checked graphically by the tangent method.30,38 The DTm
values were calculated by subtracting Tm of the free nucleic acid
from Tm of the complex. Every DTm value here reported was the
average of at least two measurements. The error in DTm is
ꢂ0.5 1C.
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Conflicts of interest
There are no conflicts to declare.
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2018, 14, 1051–1086; (b) M. Hranjec, K. Starcevic, I. Piantanida,
We acknowledge the financial support of the Croatian Science
Foundation (grants No. IP-2013-11-9310, IP-2018-01-3298 and
IP-2018-01-5475).
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