Chemistry - A European Journal p. 1403 - 1409 (2014)
Update date:2022-08-29
Topics:
Winkler, Christoph K.
Clay, Dorina
Entner, Marcello
Plank, Markus
Faber, Kurt
To develop a nicotinamide-independent single flavoenzyme system for the asymmetric bioreduction of C=C bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. Six highly potent, cheap, and commercially available co-substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even superior to, those obtained with traditional two-enzyme nicotinamide adenine dinucleotide phosphate (NAD(P)H)-recycling systems.
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