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255
higher cmcs than that of Isophol16-PC or the compounds, which
formed lamellar phases in water dispersions, were less active.
The most active compound, Isophol16-PC, may be a promis-
ing candidate for the treatment of Acanthamoeba infections. Its
trophocidal activities against studied Acanthamoeba spp. are better
in comparison with HPC and chemical structures and physico-
Therefore, Isophol16-PC may be suitable for topical administration
because the HPC shows lower toxicity against human keratinocytes
than chlorhexidine, which is a standard drug used for treatment of
Acanthamoeba keratitis (Walochnik et al., 2009).
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Acknowledgments
We thank the NMR laboratory, Faculty of Pharmacy, Come-
nius University in Bratislava for allowing us to conduct the NMR
experiments. Further we thank the management of HPL (Ltd.) for
laboratory space for testing the amoebicidal activities. This work
was supported by Grants VEGA 1/0229/10, VEGA 1/0266/10, VEGA
1/0600/11, VEGA 1/0796/12, UK/243/2011.
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