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Analytical Chemistry
2
+
CIALP (0.89 unit/mL activated by 25 nM Mg and 2.5 nM
(8) Clarke, J.; Wu, H.-C.; Jayasinghe, L.; Patel, A.; Reid, S.;
Bayley, H. Nat. Nanotechnol. 2009, 4, 265.
2+
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9
Zn ) to the cis side. (g-j) The normalized histogram plots of
the residual current at different times. (k) The concentration
variations of APs and adenosine during the hydrolysis
process. Averaged adenosine concentration was calculated by
the formula: 7.04 - ([ATP] + [ADP] + [AMP]). (l) The scatter
plot of all events during enzyme-catalyzing transformation.
The hydrolysis was conducted in solution of 1 M KCl, 10 mM
Tris-HCl, pH=7.5, at 20 °C. The applied voltage was 140 mV.
(
9) Derrington, I. M.; Butler, T. Z.; Collins, M. D.; Manrao, E.;
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5
(
9
CONCLUSIONS
(
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
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5
5
5
5
5
5
5
6
0
1
2
3
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6
7
8
9
0
1
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6
7
8
9
0
BR-nanopore assay here described is a single-molecule
approach to detect bioenergetic molecules and their
transformations, which has many potential advantages: (i)
the high selectivity of BR allows BR-nanopore to
effectively capture and identify ATP, ADP and AMP; (ii) It
is label-free, no amplification and no modification so does
not suffer from other interference; (iii) the high-
resolution real-time signals for ATP, ADP and AMP
enable us to directly read out their information; (iv) the
ability for simultaneous rapid monitoring of ATP, ADP
and AMP is hard to achieve by other methods. Although
simultaneous analyzing bioenergy-related molecules and
their transformation dynamics has been demonstrated,
BR-nanopore strategy is broadly useful to any involved-
phosphate biological processes and application, for
H. S. J. Am. Chem. Soc. 2018, 140, 14224-14234.
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ChemBioChem 2005, 6, 1875-1881.
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Bioelectron. 2014, 53, 453-458.
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D.; Mast, J.; Maglia, G. Sci. Adv. 2015, 1, e1500905.
22) Ying, Y. L.; Wang, H. Y.; Sutherland, T. C.; Long, Y.-T.
Small 2011, 7, 87-94.
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(
23,31
example, synthetic DNA sequencing method,
detecting
(
3
2-34
phosphorylation of DNA/RNA and protein
.
Bader, J. S.; Bemben, L. A.; Berka, J.; Braverman, M. S.; Chen, Y.-
J.; Chen, Z.; et al. Nature 2005, 437, 376.
ASSOCIATED CONTENT
Supporting Information
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Trézéguet, V.; Lauquin, G. J.-M.; Brandolin, G. Nature 2003, 426,
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310.
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This material is available free of charge via the Internet at
http://pubs.acs.org. Experimental details describing the
synthesis, characterization, detailed information on
molecular simulation, and analysis on hydrolysis kinetics, as
well as additional figures.
(
(27) Chetnani, B.; Das, S.; Kumar, P.; Surolia, A.; Vijayan, M.
Acta Crystallogr. D Biol. Crystallogr. 2009, 65, 312-325.
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28) Wang, Y.; Zhang, Y.; Guo, Y.; Kang, X. Sci. Rep. 2017, 7,
AUTHOR INFORMATION
Corresponding Author
183.
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29) Kang, X.; Gu, L. Q.; Cheley, S.; Bayley, H. Angew. Chem.
Int. Ed. 2005, 44, 1495-1499.
*
(30) Say, J.; Ciuffi, K.; Furriel, R. P.; Ciancaglini, P.; Leone, F. A.
Biochim. Biophys. Acta BBA-Gen. Subj. 1991, 1074, 256-262.
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Robertson, J. W. F.; Li, Z.; Russo, J. J.; Reiner, J. E.; Kasianowicz, J.
J.; et al. Sci. Rep. 2012, 2, 684.
Notes
The authors declare no competing financial interest.
ACKNOWLEDGMENT
This work was financially supported by the National Science
Foundation of China (NSFC: 21375104, 21327806 and
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32) Wu, W.; Hu, H.; Li, F.; Wang, L.; Gao, J.; Lu, J.; Fan, C.
Chem. Commun. 2011, 47, 1201-1203.
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